KINETICS OF STACKING INTERACTIONS IN FLAVIN ADENINE-DINUCLEOTIDE FROM TIME-RESOLVED FLAVIN FLUORESCENCE
KINETICS OF STACKING INTERACTIONS IN FLAVIN ADENINE-DINUCLEOTIDE FROM TIME-RESOLVED FLAVIN FLUORESCENCE
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DOI：
10.1111/j.1751-1097.1984.tb04640.x
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发表时间：
1984-01-01
影响因子：
3.3
通讯作者：
VISSER, AJWG
中科院分区：
文献类型：
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作者：
VISSER, AJWG
用亚纳秒分辨荧光仪测定了FAD荧光的时间依赖性。与FMN的荧光衰减相反，FAD的荧光衰减是非指数的。FAD的时间依赖性荧光可以通过假设基态的闭合和开放构象之间的平衡来解释。分子内复合物在激发态的折叠速率常数和荧光寿命可以通过观察到的荧光衰减来评估。将FAD测定结果与早期的NADH测定结果进行比较。
The time dependence of the fluorescence of FAD was measured with a subnanosecond-resolving fluorometer. In contrast to the fluorescence decay of FMN, the decay of FAD was proved to be nonexponential. The time-dependent fluorescence of FAD can be interpreted by assuming an equilibrium between closed and open conformers in the ground state. The rate constant for folding in the excited state and the fluorescence lifetime of the intramolecular complex could be evaluated from analysis of the observed fluorescence decay. The results on FAD were compared to those of NADH obtained earlier.