Oxidation of lipids in low density lipoprotein particles
Oxidation of lipids in low density lipoprotein particles
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DOI：
10.1080/10715769800300061
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发表时间：
1998-01-01
影响因子：
3.3
通讯作者：
Niki, E
中科院分区：
文献类型：
--
作者：
Noguchi, N;Numano, R;Niki, E
为了进一步了解低密度脂蛋白(LDL)颗粒中脂质氧化的机理和动力学，特别是为了阐明低密度脂蛋白颗粒中摄氧量和产物之间的物质平衡，以及对低密度脂蛋白颗粒中核心胆固醇酯和外单分子层磷脂酰胆碱氧化的相对敏感性。研究发现，共轭双烯或总过氧物不能解释相当大的摄氧量。用过氧化氢还原三苯基膦或二苯基吡喃基膦生成的氧化膦测定总过氧化值。胆固醇和磷脂酰胆碱是低密度脂蛋白氧化过程中形成的主要过氧化产物，但它们占总过氧化产物的60%左右。胆固醇也被氧化了，但它的氧化只有在反应的后期才显著。研究还发现，在低密度脂蛋白颗粒中，胆固醇酯相对于磷脂酰胆碱的氧化性大于在均相溶液中的氧化性，这与低密度脂蛋白颗粒的物理性质有关。
This study was undertaken to understand further the mechanisms and dynamics of the oxidation of lipids in low density lipoprotein (LDL) particles, aiming specifically at elucidating the material balance between oxygen uptake and products found and also the relative susceptibilities to oxidation of cholesteryl ester in the core and phosphatidylcholine in the outer monolayer in the LDL particles. It was found that considerable amount of oxygen uptake could not be accounted for by conjugated diene or total peroxides. Total peroxide was measured from the phosphine oxide formed from triphenylphosphine or diphenylpyrenylphosphine by reduction of peroxides. Cholesteryl ester hydroperoxides and phosphatidylcholine hydroperoxides were the major peroxides formed in LDL oxidation, but they accounted for about 60% of total peroxide. Cholesterol was also oxidized, but its oxidation was significant only at the later stages of the reaction. It was also found that the oxidizability of cholesteryl ester relative to phosphatidylcholine was larger within the LDL particle than in homogeneous solution and this was interpreted in the context of the physical properties of LDL particle.