Analysis of the floral transcriptome uncovers new regulators of organ determination and gene families related to flower organ differentiation in Gerbera hybrida (Asteraceae)
Analysis of the floral transcriptome uncovers new regulators of organ determination and gene families related to flower organ differentiation in Gerbera hybrida (Asteraceae)
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DOI：
10.1101/gr.3043705
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发表时间：
2005-04-01
期刊：
影响因子：
7
通讯作者：
Elomaa, P
中科院分区：
文献类型：
--
作者：
Laitinen, RAE;Immanen, J;Elomaa, P
菊科植物复合花序的发育具有传统模式植物不能研究的特征。在非洲菊中，花序由形态上不同类型的花紧密地包装成花头（头状花序）组成。单个花器官如冠毛（萼片）发育特化，雄蕊在边缘花中败育，花瓣和花药为融合结构，子房位于其他花器官的下位。这些特性使非洲菊成为比较研究的有益目标。在这里，我们报告的分析，非洲菊EST数据库包含16,994个cDNA序列。将这些序列与所有植物肽序列进行比较，发现非洲菊有1656个独特序列在植物界其他地方未发现。基于EST数据库，我们构建了一个包含9000个探针的cDNA微阵列，并利用它来鉴定花特异性基因和丰富表达的标记基因的花柄，冠毛，雄蕊和花瓣发育。我们的分析揭示了几个调控基因与花器官发育的推定功能。我们还能够关联的花器官分化的丰富和特异性表达的基因。非洲菊是一种异交物种，基因发现的遗传方法并不容易接受。然而，在基因转移的帮助下，反向遗传学是非常有用的。我们在这里展示了非洲菊微阵列作为一种可靠的新工具，用于识别与特定生物学问题相关的新基因和大规模基因表达分析的可用性。
Development of composite inflorescences in the plant family Asteraceae has features that cannot be studied in the traditional model plants for flower development. In Gerbera hybrida, inflorescences are composed of morphologically different types of flowers tightly packed into a flower head (capitulum). Individual floral organs such as pappus bristles (sepals) are developmentally specialized, stamens are aborted in marginal flowers, petals and anthers are fused structures, and ovaries are located inferior to other floral organs. These specific features have made gerbera a rewarding target of comparative studies. Here we report the analysis of a gerbera EST database containing 16,994 cDNA sequences. Comparison of the sequences with all plant peptide sequences revealed 1656 unique sequences for gerbera not identified elsewhere within the plant kingdom. Based on the EST database, we constructed a cDNA microarray containing 9000 probes and have utilized it in identification of flower-specific genes and abundantly expressed marker genes for flower scape, pappus, stamen, and petal development. Our analysis revealed several regulatory genes with putative functions in flower-organ development. We were also able to associate a number of abundantly and specifically expressed genes with flower-organ differentiation. Gerbera is an outcrossing species, for which genetic approaches to gene discovery are not readily amenable. However, reverse genetics with the help of gene transfer has been very informative. We demonstrate here the usability of the gerbera microarray as a reliable new tool for identifying novel genes related to specific biological questions and for large-scale gene expression analysis.