iTRAQ-Based Quantitative Proteomic Analysis of Duck Eggshell During Biomineralization
iTRAQ-Based Quantitative Proteomic Analysis of Duck Eggshell During Biomineralization
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基于 iTRAQ 的鸭蛋壳生物矿化过程中的定量蛋白质组分析
DOI：
10.1002/pmic.201900011
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发表时间：
2019
期刊：
影响因子：
3.4
通讯作者：
Hou Zhuo Cheng
中科院分区：
文献类型：
--
作者：
Zhu Feng;Zhang Fan;Hincke Maxwell;Yin Zhong Tao;Chen Si Rui;Yang Ning;Hou Zhuo Cheng
鸟蛋是自然界中最快的钙化过程，也是生物矿化的一个明显例子，因此鸟蛋是一个有价值的钙化生物矿化模型。本研究首次采用iTRAQ MS/MS检测和研究了：1）鸭蛋壳整体蛋白质组; 2）鸭蛋壳内外部分蛋白质组的区域差异。新的参考蛋白质数据集使我们能够识别179个蛋壳蛋白质，而不仅仅是使用当前版本的Ensembl鸭子注释。在整个鸭蛋壳蛋白质组中，共鉴定出484种蛋白质。还鉴定了28种参与蛋壳形成的未知功能的新蛋白质。在鉴定的蛋壳蛋白中，54种蛋白质与整体完整的蛋壳（正常排出的蛋壳）相比，在内部部分矿化的蛋壳（排卵后16小时获得）之间显示出差异丰度。至少有64种丰富的基质蛋白质是4种不同的家养鸟类（鸡，鸭，鹌鹑，火鸡）和斑胸草雀的蛋壳所共有的。本研究为鸟类蛋壳蛋白质组学研究提供了新的资源，并丰富了蛋壳基质蛋白质的研究内容，有助于更深入地了解钙质生物矿化过程。
The avian egg is a valuable model for the calcitic biomineralization process as it is the fastest calcification process occurring in nature and is a clear example of biomineralization. In this study, iTRAQ MS/MS is used to detect and study for the first time: 1) the overall duck eggshell proteome; 2) regional differences in the proteome between the inner and outer portions of the duck eggshell. The new reference protein datasets allow us to identify 179 more eggshell proteins than solely using the current release of Ensembl duck annotations. In total, 484 proteins are identified in the entire duck eggshell proteome. Twenty‐eight novel proteins of unknown function that are involved in eggshell formation are also identified. Among the identified eggshell proteins, 54 proteins show differential abundances between the inner, partially mineralized eggshell (obtained 16 h after ovulation) compared to the overall complete eggshell (normally expulsed eggshell). At least 64 of the abundant matrix proteins are common to eggshell of 4 different domesticated bird species (chicken, duck, quail, turkey) and zebra finch. This study provides a new resource for avian eggshell proteomics, and augments the inventory of eggshell matrix proteins that will lead to a deeper understanding of calcitic biomineralization.