Effect of Chaihushugan San on expression of the Raf/mitogen-activated protein kinase/extracellular signal-regulated kinase pathway in the hippocampi of perimenopausal rats induced by immobilization stress
Effect of Chaihushugan San on expression of the Raf/mitogen-activated protein kinase/extracellular signal-regulated kinase pathway in the hippocampi of perimenopausal rats induced by immobilization stress
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DOI：
10.1016/s0254-6272(15)30123-0
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发表时间：
2015-08-15
影响因子：
2.6
通讯作者：
Li Candong
中科院分区：
文献类型：
--
作者：
Li Shengqiang;Liang Wenna;Li Candong
目的：研究柴胡舒肝散对围绝经期肝气郁结大鼠行为的影响，探讨柴胡舒肝散对固定诱导大鼠海马Raf/丝裂原活化蛋白激酶(MEK)/细胞外信号调节激酶（ERK）级联信号转导的影响。方法：52周龄雌性大鼠20只，采用随机数字表法分为模型对照组（MCG）和CSS组（CSSG），每组10只。以10周龄雌性大鼠为正常对照组（NCG）。通过测量大鼠的体重、蔗糖消耗、血清激素水平和在开放场试验（OFT）中的表现来评估CSS对固定应激大鼠的影响。采用实时定量聚合酶链反应测定CSS对海马组织中Raf1、MEK1/2和ERK1/2 mRNA表达的影响，采用Western blotting测定这些蛋白及相关磷酸化蛋白的水平，研究其分子机制。结果：LQS组围绝经期大鼠运动活性降低；减少蔗糖的消耗量；血清促肾上腺皮质激素释放激素（CRH）和皮质酮（CORT）水平升高。海马Raf/MEK/ERK级联的激活在MCG中被明显抑制，并且在CSS治疗21天后激活增加。结论：CSS对固定大鼠LQS症状有明显的缓解作用。这种作用的机制可能（至少部分）是通过逆转Raf/MEK/ERK通路的破坏来介导的。(c) 2015 jtcm。版权所有。
OBJECTIVE: We wished to study the impact of Chaihushugan San (CSS) on the behavior of perimenopausal rats with liver-Qi stagnation (LQS) and to investigate the effect of CSS on signal transduction of the Raf/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) cascade in the hippocampi of rats induced by immobilization.METHODS: Twenty 52-week-old female rats were divided into two groups by the random number table method: model control group (MCG) and CSS group (CSSG), with 10 rats in each group. Ten-week-old female rats were used as the normal control group (NCG). CSS effects were assessed using rats exposed to immobilization stress by measuring body weight and sucrose consumption, serum hormone levels, and observing performance in the open field test (OFT). Molecular mechanisms were examined by measuring the effect of CSS on expression of Raf1, MEK1/2 and ERK1/2 mRNA in hippocampi using quantitative real-time polymerase chain reaction and by measuring levels of these proteins and related phospho-proteins using Western blotting.RESULTS: Perimenopausal rats with LQS had decreased locomotor activity; reduced sucrose consumption; and increased serum levels of corticotropin releasing hormone (CRH) and corticosterone (CORT). Activation of hippocampal Raf/MEK/ERK cascade was suppressed significantly in the MCG, and activation was increased after 21 days of CSS treatment.CONCLUSION: CSS has significant effects upon relief of the symptoms of LQS in immobilization-induced rats. The mechanism underlying this action might (at least in part) be mediated by reversal of disruption of the Raf/MEK/ERK pathway. (C) 2015 JTCM. All rights reserved.