Reconstitution of the central and peripheral nervous system during salamander tail regeneration
Reconstitution of the central and peripheral nervous system during salamander tail regeneration
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DOI：
10.1073/pnas.1116738109
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发表时间：
2012-08-21
影响因子：
11.1
通讯作者：
Tanaka, Elly M.
中科院分区：
文献类型：
--
作者：
Mchedlishvili, Levan;Mazurov, Vladimir;Tanaka, Elly M.
我们表明，在Ambystoma mexicanum（蝾螈）的尾切断后，正确的数目和间距的背根神经节再生。通过移植脊髓组织和非克隆的神经球，我们表明，中央脊髓周围神经系统细胞的来源。有趣的是，黑色素细胞从皮肤中预先存在的前体迁移。最后，我们证明了移植克隆衍生的脊髓神经球可以导致所有检查的细胞类型在再生的中央脊髓重建，这表明衍生的细胞与脊髓干细胞的特性。
We show that after tail amputation in Ambystoma mexicanum (Axolotl) the correct number and spacing of dorsal root ganglia are regenerated. By transplantation of spinal cord tissue and non-clonal neurospheres, we show that the central spinal cord represents a source of peripheral nervous system cells. Interestingly, melanophores migrate from preexisting precursors in the skin. Finally, we demonstrate that implantation of a clonally derived spinal cord neurosphere can result in reconstitution of all examined cell types in the regenerating central spinal cord, suggesting derivation of a cell with spinal cord stem cell properties.