Hedgehog is required for activation of engrailed during regeneration of fragmented Drosophila imaginal discs.
Hedgehog is required for activation of engrailed during regeneration of fragmented Drosophila imaginal discs.
复制标题
DOI：
--
复制
发表时间：
1999-04
期刊：
影响因子：
4.6
通讯作者：
M. Gibson;G. Schubiger
中科院分区：
文献类型：
--
作者：
M. Gibson;G. Schubiger
手术破碎的果蝇附属器官原基（成虫盘）在体内培养的短时间内参与伤口愈合和模式调节。前腿椎间盘碎片具有特殊的调节能力，前细胞转化为后细胞并建立新的后室的能力突出了这一点。这种前/后转换违反了发育谱系的限制至关重要的正常生长和图案的光盘，从而提供了一个理想的模型，了解细胞如何改变命运在epimorphic模式的调节。在这里，我们提出的证据表明，刺猬编码的分泌信号通过激活在调节前细胞中盘根错节的后特异性转录因子来指导前/后转换。在没有hedgehog活动的情况下，前腿椎间盘碎片不能进行前/后转换，但仍然可以再生缺失的前模式元素。我们认为，刺猬独立再生前室内（称为整合）介导的位置线索编码的无翅和decapentaplegic。两者合计，我们的研究结果提供了一种新的机械解释的成虫盘模式的调节，并允许推测，类似的机制可以在其他生物体的附属物再生。
Surgically fragmented Drosophila appendage primordia (imaginal discs) engage in wound healing and pattern regulation during short periods of in vivo culture. Prothoracic leg disc fragments possess exceptional regulative capacity, highlighted by the ability of anterior cells to convert to posterior identity and establish a novel posterior compartment. This anterior/posterior conversion violates developmental lineage restrictions essential for normal growth and patterning of the disc, and thus provides an ideal model for understanding how cells change fate during epimorphic pattern regulation. Here we present evidence that the secreted signal encoded by hedgehog directs anterior/posterior conversion by activating the posterior-specific transcription factor engrailed in regulating anterior cells. In the absence of hedgehog activity, prothoracic leg disc fragments fail to undergo anterior/posterior conversion, but can still regenerate missing anterior pattern elements. We suggest that hedgehog-independent regeneration within the anterior compartment (termed integration) is mediated by the positional cues encoded by wingless and decapentaplegic. Taken together, our results provide a novel mechanistic interpretation of imaginal disc pattern regulation and permit speculation that similar mechanisms could govern appendage regeneration in other organisms.