Di(n-butyl) Phthalate Induces Vimentin Filaments Disruption in Rat Sertoli Cells: A Possible. Relation with Spermatogenic Cell Apoptosis
Di(n-butyl) Phthalate Induces Vimentin Filaments Disruption in Rat Sertoli Cells: A Possible. Relation with Spermatogenic Cell Apoptosis
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DOI：
10.1111/j.1439-0264.2010.00993.x
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发表时间：
2010-06-01
影响因子：
0.9
通讯作者：
Kurohmaru, M.
中科院分区：
文献类型：
--
作者：
Alam, M. S.;Ohsako, S.;Kurohmaru, M.
邻苯二甲酸酯已广泛用作合成聚合物的增塑剂。以往的研究表明，包括邻苯二甲酸二丁酯（DBP）在内的一些邻苯二甲酸酯类物质可诱导生精细胞凋亡，但其机制尚不清楚。目前的研究表明，支持细胞波形蛋白丝的破坏DBP管理可能与生精细胞凋亡。本组织病理学研究显示，单次经口给予500 mg/kg DBP导致生精细胞逐渐脱离生精上皮并移位，并脱落至管腔中。在DBP处理的大鼠中经常观察到以染色质凝聚为特征的变性生精细胞。在超微结构上，退化的生精细胞从他们的邻居，和支持细胞波形蛋白丝的崩溃被确认在DBP处理的大鼠。支持细胞培养物显示细胞质中空泡的数量和大小增加。与体内实验一致，波形蛋白细丝清楚地显示出在体外暴露于DBP的支持细胞中逐渐塌陷。这些在体内和体外实验表明，DBP诱导的支持细胞波形蛋白丝的崩溃可能会导致生精细胞的脱离，然后脱离的细胞可能会发生凋亡，因为支持细胞提供的支持和营养的损失。
Phthalate esters have been extensively used as a plasticizer of synthetic polymers. Previous studies have revealed that some phthalate esters including di(n-butyl) phthalate (DBP) induce spermatogenic cell apoptosis, although its mechanism is not yet clear. The present study describes that disruption of Sertoli cell vimentin filaments by DBP administration may relate to spermatogenic cell apoptosis. The present histopathological study revealed that a single oral administration of 500 mg/kg DBP caused progressive detachment and displacement of spermatogenic cells away from the seminiferous epithelium and sloughing of them into the lumen. Degenerative spermatogenic cells characterized by chromatin condensation were frequently observed in DBP-treated rats. Ultrastructurally, the degenerative spermatogenic cells were separated from their neighbours, and a collapse of Sertoli cell vimentin filaments was recognized in DBP-treated rats. Sertoli cell cultures showed the increased number and size of vacuoles in their cytoplasm. In agreement with the in vivo experiment, vimentin filaments clearly showed a gradual collapse in DBP-exposed Sertoli cells in vitro. These in vivo and in vitro experiments indicate that DBP-induced collapse of Sertoli cell vimentin filaments may lead to detachment of spermatogenic cells, and then detached cells may undergo apoptosis because of loss of the support and nurture provided by Sertoli cells.