Concentration of Microbial Populations from Aquatic Environments by Vortex Flow Filtration
Concentration of Microbial Populations from Aquatic Environments by Vortex Flow Filtration
复制标题
通过涡流过滤浓缩水生环境中的微生物种群
DOI：
10.3354/meps080101
复制
发表时间：
1992
影响因子：
2.5
通讯作者：
J. Paul
中科院分区：
文献类型：
--
作者：
Sunny C. Jiang;J. Thurmond;S. Pichard;J. Paul
涡流过滤用于浓缩来自淡水、河口、沿海和寡营养海洋环境的颗粒DNA、叶绿素a和细菌。使用具有 100 kD 或 0.45 pm 过滤器的 Membrex 基准系统和具有 100 kD 过滤器的大型 Pacesetter 系统。 Benchmark 系统非常适合处理 100 m1 至 50 1 水，而 Pacesetter 则设计用于处理 20 1 至数百升的水量。配备 100 kD 过滤器的基准系统对颗粒 DNA、叶绿素 a 和细菌的平均回收率分别为 81.7%、83.3% 和 80.5%。 Pacesetter系统对颗粒DNA、叶绿素a和细菌的回收效率分别为38.0%、38.1%和43.6%。 Pacesetter 的过滤率为 66.5 f 10 1 h-'，配备 100 k D 过滤器的 Benchmark 系统的过滤率为 7.8 1 h"。我们的结果表明，Benchmark 系统适合需要使用少量水进行高效率回收的定量研究，而 Pacesetter 更适合需要从大量水中浓缩微生物种群以进行遗传和分子系统发育研究的定性研究。
Vortex Flow Filtrat~on was used to concentrate particulate DNA, chlorophyll a and bacteria from freshwater, estuarine, coastal and oligotrophic marine environments. A Membrex Benchmark System with either a 100 kD or a 0.45 pm filter and a large-scale Pacesetter System with a 100 kD filter were used. The Benchmark System is ideal for processing 100 m1 to 50 1 of water, while the Pacesetter is designed for working with volumes from 20 1 up to hundreds of liters. The Benchmark System with a 100 kD filter yielded an average recovery of 81.7, 83.3 and 80.5 % respectively for particulate DNA, chlorophyll a and bacteria. The efficiency of recovery with the Pacesetter System was 38.0 , 38.1 and 43.6 % respectively for part~culate DNA, chlorophyll a , and bacteria. The filtration rate was 66.5 f 10 1 h-' for the Pacesetter and 7.8 1 h" for the Benchmark System with the 100 k D filters. Our results indicate that the Benchmark System is suitable for quantitive studies that require a high efficiency of recovery using small volumes of water, while the Pacesetter is more suited for qualitative studies that require concentrating microbial populations from large quantities of water for genetic and molecular phylogenetic studies.