Loss of heterozygosity in human primary prostate carcinomas: a possible tumor suppressor gene at 7q31.1.
Loss of heterozygosity in human primary prostate carcinomas: a possible tumor suppressor gene at 7q31.1.
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发表时间：
1994-12
期刊：
影响因子：
11.2
通讯作者：
J. Zenklusen;Janet C. Thompson;P. Troncoso;J. Kagan;C. Conti
中科院分区：
文献类型：
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作者：
J. Zenklusen;Janet C. Thompson;P. Troncoso;J. Kagan;C. Conti
我们研究了人类 7q 染色体上的杂合性缺失 (LOH)，以确定人类原发性前列腺癌中假定的肿瘤抑制基因 (TSG) 的位置。样本取自德克萨斯大学 MD 安德森癌症中心通过手术切除患者的 16 例原发性前列腺癌。使用配对的正常和肿瘤 DNA 作为模板，对 7q21-qter 上的一组 14 个 CA 微卫星重复进行 PCR 扩增。研究的 16 个病例中，有 12 个在 7q 上的一个或多个位点有 LOH。使用 D7S522 在 7q31.1-7q31.2 检测到 83% 的 LOH（六个信息性病例中的五个）。 LOH 的百分比呈正态分布在 D7S522 周围。原发性前列腺癌中 LOH 的高发生率表明，7q31.1-31.2 处存在与前列腺癌发生相关的 TSG，证实了我们之前关于该位置存在 TSG 的功能证据。需要进行进一步的研究来确定这个假定的 TSG 的身份和功能。
We studied loss of heterozygosity (LOH) on human chromosome 7q to determine the location of a putative tumor suppressor gene (TSG) in human primary prostate carcinomas. Samples were obtained from 16 primary prostate carcinomas surgically removed from patients at The University of Texas M. D. Anderson Cancer Center. Paired normal and tumor DNAs were used as template for PCR amplification of a set of 14 CA microsatellite repeats on 7q21-qter. Twelve of 16 cases studied had LOH at one or more loci on 7q. Eighty-three percent LOH (five of six informative cases) was detected with D7S522 at 7q31.1-7q31.2. Percentage of LOH was normally distributed around D7S522. The high incidence of LOH in primary prostate carcinomas suggests that there is a TSG relevant to the development of prostate cancers at 7q31.1-31.2, confirming our previous functional evidence for a TSG at this location. Further research needs to be conducted to establish the identity and function of this putative TSG.