Abnormal glucocorticoid receptors in acute leukemia cells.
Abnormal glucocorticoid receptors in acute leukemia cells.
复制标题
急性白血病细胞中糖皮质激素受体异常。
作者：
R. Mccaffrey;A. Lillquist;R. Bell
在正常组织中，~ 3 H-曲安奈德（~ 3 H-TA）标记的糖皮质激素受体经DEAE层析可分离为2个组分：峰I在0.04 M盐处洗脱，峰II在0.22 M盐处洗脱。经甘油梯度离心，峰I为3.5S，峰II为8.5S。峰I与DNA结合，而峰II不结合。62例急性白血病中27例原始细胞3 H-TA结合大分子与正常组织的DEAE结合特征相同，其余35例异常。在这些情况下，在峰I区域中洗脱单个DEAE物质（30例）或在整个梯度中洗脱多个低振幅峰（5例）。在5例（研究的5例）中，异常单峰物质未能与DNA结合，而来自5例（研究的5例）的峰I物质显示正常的峰I-峰II比率，正常地与DNA结合。在3例研究病例中，异常单峰物质的S值为2-2.5S，而在5例I峰-II峰比值正常的病例中，S值分别为3.5S和8.5S。我们推测，这些白血病与异常粘合剂的特点不能响应糖皮质激素治疗。
In normal tissues, 3H-triamcinolone acetonide (3H-TA) labeled glucocorticoid receptors can be resolved into 2 components by DEAE chromatography: peak I elutes at 0.04 M salt and peak II at 0.22 M salt. By glycerol gradient centrifugation, peak I is 3.5S and peak II is 8.5S. Peak I binds to DNA, while peak II does not. Blast cell 3H-TA-binding macromolecules in 27 of 62 cases of acute leukemia had DEAE binding characteristics identical to those of normal tissues; the remaining 35 cases were abnormal. In these cases there was either a single DEAE species eluting in the peak I area (30 cases) or multiple low-amplitude peaks eluting across the entire gradient (5 cases). The abnormal single peak material failed to bind to DNA in 5 cases (of 5 studied), whereas peak I material from 5 cases (of 5 studied), showing normal peak I-peak II ratios, bound normally to DNA. In 3 cases (of 3 studied), the abnormal single peak material had an S value of 2-2.5S, whereas in 5 cases with normal peak I-peak II ratios, the S values were 3.5S and 8.5S, respectively. We hypothesize that those leukemias with abnormal binder characteristics cannot respond to glucocorticoid therapy.