Photocrosslinkable chitosan hydrogels functionalized with the RGD peptide and phosphoserine to enhance osteogenesis.
Photocrosslinkable chitosan hydrogels functionalized with the RGD peptide and phosphoserine to enhance osteogenesis.
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DOI：
10.1039/c6tb01154c
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发表时间：
2016-08-21
期刊：
影响因子：
--
通讯作者：
Lee M
中科院分区：
文献类型：
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作者：
Kim S;Cui ZK;Fan J;Fartash A;Aghaloo TL;Lee M
来源于天然存在的聚合物的水凝胶是用于组织工程的有吸引力的基质。在这里，我们报告了一种生物功能性水凝胶，用于骨再生的特定用途，通过引入Arg-Gly-Asp（RGD）-含有细胞粘附基序和磷酸化丝氨酸残基，这是普遍存在于天然骨细胞外基质和已知的促进成骨，通过增强细胞-基质相互作用和羟基磷灰石成核，到光聚合甲基丙烯酸乙二醇壳聚糖（MeGC）。将磷酸丝氨酸掺入MeGC水凝胶中增加了水凝胶在其表面上成核矿物的能力。RGD掺入通过支持包封在水凝胶中的骨髓基质细胞（BMSC）的附着、扩散和增殖来增强细胞-基质相互作用。此外，共同修饰的MeGC水凝胶与RGD和磷酸丝氨酸协同增加成骨分化的封装骨髓基质细胞在体外。在小鼠颅骨缺损模型中进一步证实了改性水凝胶的骨愈合能力。这些发现表明具有特定微环境的有前途的水凝胶平台适合促进骨生成用于临床骨修复。
Hydrogels derived from naturally occurring polymers are attractive matrix for tissue engineering. Here, we report a biofunctional hydrogel for specific use in bone regeneration by introducing Arg-Gly-Asp (RGD)-containing cell adhesive motifs and phosphorylated serine residues, which are prevalent in native bone extracellular matrix and known to promote osteogenesis by enhancing cell-matrix interactions and hydroxyapatite nucleation, into photopolymerizable methacrylated glycol chitosan (MeGC). Incorporation of phosphoserine into MeGC hydrogels increased the ability of the hydrogels to nucleate mineral on their surfaces. RGD incorporation enhanced cell-matrix interactions by supporting attachment, spreading, and proliferation of bone marrow stromal cells (BMSCs) encapsulated in the hydrogels. Moreover, co-modification of MeGC hydrogels with RGD and phosphoserine synergistically increased osteogenic differentiation of encapsulated BMSCs in vitro. The bone healing capacity of the modified hydrogels was further confirmed in a mouse calvarial defect model. These findings suggest a promising hydrogel platform with a specific microenvironment tailored to promote osteogenesis for clinical bone repair.