A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana
A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana
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DOI：
10.1016/j.molp.2014.10.008
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发表时间：
2015-04-06
期刊：
影响因子：
27.5
通讯作者：
Fukuda, Hiroo
中科院分区：
文献类型：
--
作者：
Kondo, Yuki;Fujita, Takashi;Fukuda, Hiroo
在维管发育过程中，原形成层和形成层细胞形成木质部和韧皮部细胞。由于血管组织埋藏较深，因此很难对血管发育过程进行详细分析。在这里，我们建立了一个新的体外实验系统，在这个系统中，用糖原合成酶激酶3蛋白的抑制剂bikinin诱导拟南芥叶盘培养中的维管发育。转录组分析表明，叶盘中的叶肉细胞同步转化为原形成层细胞，然后分化为管状细胞。表达原形成层细胞标记TDRpro：GUS和TDRpro：YFP的植物叶盘培养物可用于原形成层细胞形成的时空可视化。用TdR突变体和TDIF进一步分析表明，关键信号TDIF-TDR-GSK3s调控叶盘培养的木质部分化。这种新的培养体系可以与利用拟南芥丰富的物质资源(包括细胞标记系和突变体)的分析相结合，从而为分析木质部细胞分化提供了强有力的工具。
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll cells in leaf disks synchronously turn into procambial cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial cell markers TDRpro: GUS and TDRpro: YFP can be used for spatiotemporal visualization of procambial cell formation. Further analysis with the tdr mutant and TDIF (tracheary element differentiation inhibitory factor) indicates that the key signaling TDIF-TDR-GSK3s regulates xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including cell-marker lines and mutants, thus offering a powerful tool for analyzing xylem cell differentiation.