Probing the activation of the replicative origin of broad host-range plasmid R1162 with Tus, the E.coli anti-helicase protein.
Probing the activation of the replicative origin of broad host-range plasmid R1162 with Tus, the E.coli anti-helicase protein.
复制标题
使用大肠杆菌抗解旋酶蛋白 Tus 探测广泛宿主范围质粒 R1162 复制起点的激活。
DOI：
10.1093/nar/19.19.5379
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发表时间：
1991
影响因子：
14.9
通讯作者：
Meyer,RJ
中科院分区：
文献类型：
--
作者：
Zhou,HS;Byrd,C;Meyer,RJ
大肠杆菌蛋白是一种抗解旋酶，参与染色体复制的终止。该蛋白的结合位点 ter 被克隆到广泛宿主范围的质粒 R1162 的衍生物中。 ter位点引起含有Tus的细胞提取物中质粒复制叉运动的定向特异性终止。构建质粒，以便在包含iteron的起始结构域侧翼有两个用于起始R1162复制的位点。在这些质粒中，含有 iteron 的结构域内富含 AT 区域旁边的位点更加活跃。此外，当ter被放置在更活跃的位点和迭代子之间时，从该位点开始的复制被特异性抑制。这些数据支持一种模型，该模型可让必需的质粒编码解旋酶进入同向重复序列的一侧，并主要朝远离这些重复序列的一个方向移动，以激活 DNA 复制的起始位点。
TheE.coliTus protein is an anti-helicase involved in the termination of chromosome replication. The binding site for this protein, ter, was cloned into derivatives of the broad host-range plasmid R1162. The ter site caused the orientation-specific termination of plasmid replication fork movement in cell extracts containing Tus.Plasmids were constructed so that two sites for initiation of R1162 replication flanked the iteron-containing domain of the origin. In these plasmids, the site next to the AT-rich region within the iteron-containing domain was more active. In addition, when ter was placed between the more active site and the iterons, initiation of replication from this site was specifically inhibited. The data support a model for entry of the essential, plasmid-encoded helicase at one side of the direct repeats, and for its movement primarily in one direction away from these repeats to activate the initiation sites for DNA replication.