Cell‐free translation product containing corticotropin and β‐endorphin encoded by messenger RNA from anterior lobe and intermediate lobe of bovine pituitary
Cell‐free translation product containing corticotropin and β‐endorphin encoded by messenger RNA from anterior lobe and intermediate lobe of bovine pituitary
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牛垂体前叶和中叶信使 RNA 编码的含有促肾上腺皮质激素和 β-内啡肽的无细胞翻译产物
DOI：
10.1016/0014-5793(77)81067-3
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发表时间：
1977
期刊：
影响因子：
3.5
通讯作者：
S. Numa
中科院分区：
文献类型：
--
作者：
S. Nakanishi;A. Inoue;S. Taii;S. Numa
促肾上腺皮质激素（ACTH）在垂体中产生，是一种单肽，大约有1，wt 4500。我们之前使用无细胞蛋白合成系统的工作表明，牛垂体前叶ACTH mRNA翻译的初始直接产物约为35000[1]。这一结果引起了人们对ACTH大分子形式的额外部分的生物学作用的兴趣。既往研究表明，ACTH和/3-LPH在相同的生理或病理情况下在同一垂体细胞中产生和储存，并同时释放[2-5]。此外，对肽结构的研究表明，几种生物活性肽可以从相同的前体制备；7- lph、/3-MSH、内啡肽和蛋氨酸-脑啡肽是由/3-LPH产生的[6,7]，而a-MSH和CLIP是由ACTH[8]形成的。因此，我们似乎可以假设ACTH和/3-LPH是由同一mRNA编码的共同前体在翻译后裂解形成的。ACTH的另一个有趣的特征是a-MSH和CLIP的发生取决于其前体的解剖定位；促肾上腺皮质激素，缩写：促肾上腺皮质激素；促肾上腺皮质激素氨基酸序列残基为1-24的合成肽ACTH-(1-24)；#液体变阻器,t3-1ipotropin;3、-LPH， 3、-脂促素，c~-MSH， a-促黑素，#-MSH，/3-促黑素；促肾上腺皮质激素样中间叶肽；免疫球蛋白G IgG在中间叶合成时转化为a-MSH和CLIP，而ACTH在前叶合成时释放为完整的ACTH[9]。尽管这种差异被认为是由于特定肽酶的存在，但来自中间叶的ACTH mRNA的无细胞翻译产物与来自前叶的ACTH mRNA的无细胞翻译产物仍有待研究。在本研究中，利用无细胞蛋白合成系统提供的证据表明，ACTH mRNA的翻译产物实际上含有/3-内啡肽序列，并且该产物不仅由来自牛垂体前叶的mRNA编码，也由来自牛垂体中间叶的mRNA编码。
Corticotropin (ACTH), which is produced in the pituitary, is a single polypeptide with approx, tool. wt 4500. Our previous work with the use of a cell-free protein-synthesizing system has demonstrated that the initial, direct product of translation of ACTH mRNA from the anterior lobe of bovine pituitary has approx, mol. wt 35 000 [1]. This result has aroused interest in the biological role of the extra-portion irt the large molecular form of ACTH. Previous investigations have suggested that ACTH and/3-LPH are produced and stored in the same pituitary cell and released together in the same physiological or pathological situations [2-5]. Furthermore, studies on peptide structures have indicated that several biologically active peptides can be elaborated from the same precursor; 7-LPH,/3-MSH, endorphins and methionine-enkephalin are derived from/3-LPH [6, 7], while a-MSH and CLIP are formed from ACTH [8]. Thus, it appeared plausible to assume that ACTH and/3-LPH are formed by post-translational cleavage of a common precursor encoded by the same mRNA. Another interesting feature of ACTH is the fact that the occurrence of a-MSH and CLIP depends on the anatomical localization of their precursor; ACTH, Abbreviations: ACTH, corticotropin; ACTH-(1-24), synthetic peptide with residues 1-24 of the amino acid sequence of corticotropin;#-LPH, t3-1ipotropin; 3,-LPH, 3,-lipotropin, c~-MSH, a-melanotropin;#-MSH,/3-melanotropin; CLIP, corticotropin-like intermediate lobe peptide; IgG, immunoglobulin G when synthesized in the intermediate lobe, is converted to a-MSH and CLIP, while ACTH, when synthesized in the anterior lobe, is released as intact ACTH [9]. Although this difference is believed to be due to the presence of specific peptidases, the cell-free translation product of ACTH mRNA from the intermediate lobe remained to be examined in comparison with that of ACTH mRNA from the anterior lobe. In the present investigation, evidence has been provided by the use of a cell-free protein-synthesizing system to indicate that the translation product of ACTH mRNA in fact contains the/3-endorphin sequence and that this product is encoded by mRNA not only from the anterior lobe but also from the intermediate lobe of bovine pituitary.