Receptors and neurons for fly odors in Drosophila
Receptors and neurons for fly odors in Drosophila
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DOI：
10.1016/j.cub.2007.02.043
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发表时间：
2007-04-03
期刊：
影响因子：
9.2
通讯作者：
Carlson, John R.
中科院分区：
文献类型：
--
作者：
van Naters, Wynand van der Goes;Carlson, John R.
关于果蝇配偶识别的分子和细胞基础知之甚少[1]。我们系统地研究了果蝇触角上的嗅觉感受器神经元（ORN）的三种主要感受器之一，通过电生理学分析。我们发现，没有强烈的食物气味，但所有的反应苍蝇气味。毛状感器的两个亚型含有对顺式乙酸乙烯酯（cVA）（一种在交配期间从雄性转移到雌性的抗催情信息素）响应的ORN [2-4]。所有的蜘蛛感器产量响应男性提取物;一个子集产量响应处女女性提取物以及。因此，男性可以从处女女性之间的活动，他们引起的ORN人口。然后，我们通过使用体内表达系统[9]系统地测试了在毛状感器[8]中表达的气味受体（Or）基因家族[5-7]的所有成员[9]。在这个系统中，有四种受体对苍蝇气味作出反应：两种对雄性和处女雌性的提取物作出反应，两种对cVA作出反应。我们提出了一个模型，描述了这些受体可能被用于男性区分合适的不合适的交配伙伴，通过一个简单的逻辑。
Remarkably little is known about the molecular and cellular basis of mate recognition in Drosophila [1]. We systematically examined the trichoid sensilla, one of the three major types of sensilla that house olfactory receptor neurons (ORNs) on the Drosophila antenna, by electrophysiological analysis. We find that none respond strongly to food odors but that all respond to fly odors. Two subtypes of trichold sensilla contain ORNs that respond to cis-vaccenyl acetate (cVA), an anti-aphrodisiac pheromone transferred from males to females during mating [2-4]. All trichoid sensilla yield responses to a male extract; a subset yield responses to a virgin-female extract as well. Thus, males can be distinguished from virgin females by the activity they elicit among the trichoid ORN population. We then systematically tested all members of the Odor receptor (Or) gene family [5-7] that are expressed in trichold sensilla [8] by using an in vivo expression system [9]. Four receptors respond to fly odors in this system: Two respond to extracts of both males and virgin females, and two respond to cVA. We propose a model describing how these receptors might be used by a male to distinguish suitable from unsuitable mating partners through a simple logic.