Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays.
Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays.
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低剂量率伽马射线长期照射后小鼠肝组织钙网蛋白上调
DOI：
10.1371/journal.pone.0182671
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ding D
中科院分区：
文献类型：
--
作者：
Yi L;Hu N;Yin J;Sun J;Mu H;Dai K;Ding D
低剂量或低剂量率电离辐射对正常组织的生物学效应已引起人们的关注。在前人研究的基础上，我们观察了C57 BL/6 J小鼠接受<50、50-500和500-1000 μ戈伊/h ~（137）Cs照射180 d后肝组织的形态学变化。结果发现，随着照射剂量率的增加，肝组织的病理变化更加明显。此外，使用蛋白质组学方法分析肝组织中的差异蛋白质表达。在辐射组的2D凝胶分析中，与匹配组相比，69个蛋白质的表达变化≥ 1.5倍。根据表达变化≥2.5倍，选择了23种蛋白质，其中22种蛋白质对生物信息学和蛋白质指纹分析有意义。这些分子与细胞骨架过程、细胞代谢、生物防御、线粒体损伤、解毒和肿瘤发生有关。实时荧光定量PCR和免疫印迹分析结果表明，钙网蛋白（CRT）在低剂量照射组中表达上调，提示CRT可能与低剂量率电离辐射对小鼠肝脏的应激反应有关，低剂量率电离辐射可能具有致癌风险。CRT蛋白可能成为低剂量或低剂量率电离辐射预警生物标志物的潜在候选者。然而，其潜在机制需要进一步研究。
The biological effects of low-dose or low-dose-rate ionizing radiation on normal tissues has attracted attention. Based on previous research, we observed the morphology of liver tissues of C57BL/6J mice that received <50, 50–500, and 500–1000 μGy/h of 137Cs radiation for 180 d. We found that the pathological changes in liver tissues were more obvious as the irradiation dose rates increased. Additionally, differential protein expression in liver tissues was analyzed using a proteomics approach. Compared with the matched group in the 2D gel analysis of the irradiated groups, 69 proteins had ≥ 1.5-fold changes in expression. Twenty-three proteins were selected based on ≥2.5-fold change in expression, and 22 of them were meaningful for bioinformatics and protein fingerprinting analysis. These molecules were relevant to cytoskeleton processes, cell metabolism, biological defense, mitochondrial damage, detoxification and tumorigenesis. The results from real-time PCR and western blot (WB) analyses showed that calreticulin (CRT) was up-regulated in the irradiated groups, which indicates that CRT may be relevant to stress reactions when mouse livers are exposed to low-dose irradiation and that low-dose-rate ionizing radiation may pose a cancer risk. The CRT protein can be a potential candidate for low-dose or low-dose-rate ionizing radiation early-warning biomarkers. However, the underlying mechanism requires further investigation.
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影响因子：
3.7
作者：
Rosenstierne MW;McLoughlin KS;Olesen ML;Papa A;Gardner SN;Engler O;Plumet S;Mirazimi A;Weidmann M;Niedrig M;Fomsgaard A;Erlandsson L
通讯作者：
Erlandsson L
影响因子：
168.9
作者：
Rosell, Rafael;Bivona, Trever G.;Karachaliou, Niki
通讯作者：
Karachaliou, Niki
DOI：
10.7868/s0869803116010136
发表时间：
2016-01-01
期刊：
Radiatsionnaya Biologiya Radioekologiya
影响因子：
--
作者：
Rybkina, V. L.;Azizova, T. V.
通讯作者：
Azizova, T. V.
低剂量的电离辐射下的癌症风险：原子弹幸存者的人工神经网络推断。
影响因子：
2
作者：
Sasaki MS;Tachibana A;Takeda S
通讯作者：
Takeda S
影响因子：
7.5
作者：
Chen, Qifeng;Fang, Xiaoming;Fang, Xudong
通讯作者：
Fang, Xudong