Intracellular Imaging of Cesium Distribution in Arabidopsis Using Cesium Green

Intracellular Imaging of Cesium Distribution in Arabidopsis Using Cesium Green
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DOI:
10.1021/am5009453
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发表时间:
2014-06-11
影响因子:
9.5
通讯作者:
Ariga, Katsuhiko
Ariga, Katsuhiko
中科院分区:
材料科学2区
文献类型:
--
作者:
Akamatsu, Masaaki;Komatsu, Hirokazu;Ariga, Katsuhiko

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福岛第一核电站事故是东日本大地震最严重的不利影响之一,伴随着大量放射性物质,包括铯-137释放到环境中。在之前的报告中,我们开发并提出了一种铯(Cs)荧光探针“铯绿色”,它可以通过喷射铯绿色探针的酒精溶液来检测碳酸铯颗粒。在本文中,该探针的传感活性,其选择性(通过使用光电管方法)和其应用程序检测微米级铯粒子进行了研究。铯绿色也被评估其在植物细胞成像的Cs定位在拟南芥。铯绿色使含铯粒子和植物中所含铯的高分辨率铯成像成为可能。
The accident at the Fukushima Daiichi nuclear power plant, which was one of the most serious adverse effects of the Great East Japan Earthquake, was accompanied by the release of a large quantity of radioactive materials including Cs-137 to the environment. In a previous report, we developed and proposed a cesium (Cs) fluorescent probe, "Cesium Green", that enables the detection of cesium carbonate particles by spraying an alcoholic solution of the Cesium Green probe. In this paper, the sensing activity of this probe was investigated for its selectivity (by using an optode method) and for its application to detect micrometer-sizes Cs particles. Cesium Green was also assessed for its use in plant cellular imaging of Cs localization in Arabidopsis. Cesium Green enabled high-resolution Cs imaging of Cs-containing particles and of Cs contained in plants.