Natural variation in Fatty Acid 9 is a determinant of fatty acid and protein content
Natural variation in Fatty Acid 9 is a determinant of fatty acid and protein content
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DOI：
10.1111/pbi.14222
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发表时间：
2023-11
影响因子：
13.8
通讯作者：
Zhaoming Qi;Chaocheng Guo;Haiyang Li;Hongmei Qiu;Hui Li;Cholnam Jong;Guolong Yu;Yu Zhang;Limin Hu;Xiaoxia Wu;Dawei Xin;Mingliang Yang;Chunyan Liu;Jian Lv;Xu Wang;Fanjiang Kong;Qingshan Chen
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文献类型：
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作者：
Zhaoming Qi;Chaocheng Guo;Haiyang Li;Hongmei Qiu;Hui Li;Cholnam Jong;Guolong Yu;Yu Zhang;Limin Hu;Xiaoxia Wu;Dawei Xin;Mingliang Yang;Chunyan Liu;Jian Lv;Xu Wang;Fanjiang Kong;Qingshan Chen
大豆是世界上最重要的经济作物之一，也是人类饮食中不饱和脂肪酸和蛋白质的重要来源。消费者对健康油脂的需求正在增加，预计到2050年全球植物油需求将翻一番。确定调节种子脂肪酸含量的关键基因可以促进具有增强脂肪酸谱的优质大豆品种的分子育种。在这里，我们分析了大豆种子脂肪酸含量变异的遗传结构，使用547份材料，主要包括来自中国东北的地方品种和栽培品种。通过脂肪酸谱分析、基因组重测序、群体基因组学分析和GWAS，我们确定了FA9位点的SEIPIN同源物是种子脂肪酸含量的重要贡献者。转基因和多组学分析证实，FA 9是种子脂肪酸含量的关键调节因子，对种子蛋白质和种子大小具有多效性。我们确定了两个主要的FA9单倍型在1295重测序的大豆种质，并评估其表型的影响，在田间种植的424个种质。携带FA9 H2基因的大豆总脂肪酸含量显著高于携带FA9 H1基因的大豆，而蛋白质含量显著低于携带FA9 H1基因的大豆。FA9 H2在野生大豆中不存在，但存在于13%的地方品种和26%的栽培品种中，这表明它可能是在大豆驯化后改良过程中选择的。因此，FA9代表了一种有用的遗传资源，可用于分子育种具有特定种子贮藏特性的高品质大豆品种。
Summary Soybean is one of the most economically important crops worldwide and an important source of unsaturated fatty acids and protein for the human diet. Consumer demand for healthy fats and oils is increasing, and the global demand for vegetable oil is expected to double by 2050. Identification of key genes that regulate seed fatty acid content can facilitate molecular breeding of high‐quality soybean varieties with enhanced fatty acid profiles. Here, we analysed the genetic architecture underlying variations in soybean seed fatty acid content using 547 accessions, including mainly landraces and cultivars from northeastern China. Through fatty acid profiling, genome re‐sequencing, population genomics analyses, and GWAS, we identified a SEIPIN homologue at the FA9 locus as an important contributor to seed fatty acid content. Transgenic and multiomics analyses confirmed that FA9 was a key regulator of seed fatty acid content with pleiotropic effects on seed protein and seed size. We identified two major FA9 haplotypes in 1295 resequenced soybean accessions and assessed their phenotypic effects in a field planting of 424 accessions. Soybean accessions carrying FA9 H2 had significantly higher total fatty acid contents and lower protein contents than those carrying FA9 H1 . FA9 H2 was absent in wild soybeans but present in 13% of landraces and 26% of cultivars, suggesting that it may have been selected during soybean post‐domestication improvement. FA9 therefore represents a useful genetic resource for molecular breeding of high‐quality soybean varieties with specific seed storage profiles.