Continuous cropping of soybean induced a more fluctuating fungal network and intensive pathogenic fungal interactions in a Mollisol of Northeast China
Continuous cropping of soybean induced a more fluctuating fungal network and intensive pathogenic fungal interactions in a Mollisol of Northeast China
复制标题
大豆连作导致中国东北软土中真菌网络波动更大，病原真菌相互作用更强烈
DOI：
10.1002/saj2.20069
复制
发表时间：
2020-05-01
影响因子：
2.9
通讯作者：
Wang, Guanghua
中科院分区：
文献类型：
--
作者：
Hu, Xiaojing;Liu, Junjie;Wang, Guanghua
大豆连作和轮作对土壤真菌群落的影响已被广泛研究，但对连作和轮作影响下的土壤真菌网络结构及真菌间的相互作用研究较少。本研究利用分子生态网络分析管道（MENAP），构建了大豆连作和玉米轮作条件下土壤和根际真菌生态网络。结果表明，两种耕作制度下，土壤和根际土壤的网络结构和特征存在较大差异。与CR相比，CC具有简化的土壤网络结构。在非根际土壤中，与CR相比，CC形成了更稳定的网络，抗环境干扰能力强，而在根际土壤中则相反。无论是根际土壤还是非根际土壤，CC中真菌种间的相互作用都比CR中的多。在根际土壤CC网络中发现了一些节点度最高的潜在致病模式的功能真菌，它们与潜在致病真菌之间存在正相互作用，与有益真菌之间存在负相互作用。可见，大豆连作削弱了根际土壤真菌网络结构，增强了潜在病原真菌间的正相互作用。
The effects of continuous cropping and crop rotation of soybean on soil fungal communities have been intensively studied, but we know little about the network structure and interactions among the fungal species impacted by these two cropping systems in the bulk and rhizosphere soils. In this study, through the Molecular Ecological Network Analysis Pipeline (MENAP), we constructed fungal ecological networks for bulk and rhizosphere soils under continuous cropping (CC) of soybean and cropping rotation (CR) with maize. The results showed that the network structures and characteristics were largely different between the two cropping systems in both the bulk and rhizosphere soils. Compared with the CR, the CC had simplified network structures in the bulk and rhizosphere soils. In the bulk soil, compared with the CR, CC formed a more stable network, which was resistant to environmental disturbance, but opposite trend was detected in the rhizosphere soil. More cooperative interactions among fungal species were found in CC than those in CR for both bulk and rhizosphere soils. Some functional fungi annotated to potentially pathogenic modes with highest node degree were found in CC network of rhizosphere soil, which had positive interactions with potentially pathogenic fungi and negative interactions with beneficial fungi. Taken together, continuous cropping of soybean weakened fungal network structure and strengthened the positive interactions among potentially pathogenic fungi in the rhizosphere soil.