Prognostic significance of Ku70 protein expression in patients with advanced colorectal cancer.
Prognostic significance of Ku70 protein expression in patients with advanced colorectal cancer.
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发表时间：
2005-07
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通讯作者：
Y. Komuro;Toshiaki Watanabe;Y. Hosoi;Y. Matsumoto;K. Nakagawa;N. Suzuki;H. Nagawa
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作者：
Y. Komuro;Toshiaki Watanabe;Y. Hosoi;Y. Matsumoto;K. Nakagawa;N. Suzuki;H. Nagawa
背景/目的Ku蛋白刺激DNA修复并发出损伤/应激反应信号，可能影响细胞凋亡和增殖。Ku 70在人胃癌细胞系中的表达与细胞增殖有关。然而，在大肠癌中，Ku 70表达的临床意义尚不清楚。我们研究了Ku 70在散发性结直肠癌中的表达模式及其与临床病理参数和生存率的关系。方法：我们研究了101例连续的进展期结直肠癌。在切除标本中，Ku 70蛋白染色阳性的细胞数量通过免疫组化进行评估。结果Ku 70在不同肿瘤中的表达率为4.2- 99.7%。Ku 70高表达组占52/101例（52%）。Ku蛋白表达模式与除浸润深度外的其他临床参数无显著相关性。多因素分析显示pTNM分期、病理分级和Ku 70是影响预后的重要因素。结论：本研究首次报道结直肠腺癌Ku蛋白表达增高与生存率降低相关。Ku可能是一个新的预后指标，可用于选择辅助治疗策略。
BACKGROUND/AIMS Ku protein stimulates DNA repair and signals the damage/stress responses, which may affect apoptosis and cell proliferation. The expression of Ku70 has been reported to be related to cell proliferation in a human gastric cancer cell line. However, in colorectal carcinoma, the clinical significance of Ku70 expression remains unclear. We examined the expression pattern of Ku70 in sporadic colorectal cancer and its relation to clinicopathological parameters and survival. METHODOLOGY We studied 101 consecutive cases of advanced colorectal carcinoma. In resected specimens, the number of cells stained positive for Ku70 protein was evaluated by immunohistochemistry. RESULTS The percentage of cells expressing Ku70 in different tumors ranged from 4.2-99.7%. The elevated Ku70 expression group comprised 52 of 101 cases (52%). No significant correlation was seen between Ku expression pattern and the clinical parameters except depth of invasion. pTNM stage, histopathological grade and Ku70 were significant variables for prognosis of survival in the multivariate analysis. CONCLUSIONS This is the first report correlating reduced survival with elevated expression of Ku protein in colorectal adenocarcinoma. Ku may be a new prognostic marker useful for selecting adjuvant treatment strategies.