Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii.
Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii.
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DOI：
10.4238/2014.december.18.9
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发表时间：
2014-12
期刊：
影响因子：
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通讯作者：
N. Z. Zhang;S. Y. Huang;D. Zhou;Y. Xu;J. J. He-J.;X. Zhu
中科院分区：
文献类型：
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作者：
N. Z. Zhang;S. Y. Huang;D. Zhou;Y. Xu;J. J. He-J.;X. Zhu
弓形虫是一种世界性的人类机会致病菌。研制有效的疫苗被认为是控制T.弓形虫感染然而，只有一种活疫苗可用于绵羊和山羊。因此，鉴定更有效的抗原蛋白是非常重要的。在这项研究中，我们确定了一个新的推定的钙依赖性蛋白激酶的T。弓形虫TgCDPK 6的抗原性，并进一步用生物信息学方法分析其抗原性。采用多种生物信息学方法预测了TgCDPK 6的理化特性、跨膜结构域、表位、高级结构和功能位点。在蛋白质中鉴定了26个翻译后修饰位点。二级结构分析表明，TgCDPK 6的58.35%的氨基酸暴露于溶剂界面，并且高亲水性结构域分布在氨基酸位置21-59、68-81、156-205、245-271、280-294、297-324、334-356、367-393、474-498和543-553。通过同源模建方法构建了TgCDPK 6的高级结构，并通过PROTEINS进行了验证，结果表明大部分氨基酸残基位于最有利的区域。利用这些分析，预测了10个潜在的表位。结果表明，TgCDPK 6可以作为抗T.刚地。
Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very important. In this study, we identified a novel putative calcium-dependent protein kinase of T. gondii, TgCDPK6, and further analyzed its potential antigenicity using a bioinformatic approach. The physical and chemical characteristics, transmembrane domain, epitopes, advanced structure, and functional sites of TgCDPK6 were predicted by multiple bioinformatic approaches. Twenty-six post-translational modification sites were identified in the protein. The secondary structure showed that 58.35% amino acids of TgCDPK6 are exposed to the solvent interface, and the high hydrophilic domains were distributed in amino acid positions 21-59, 68-81, 156-205, 245-271, 280-294, 297-324, 334-356, 367-393, 474-498, and 543-553. The advanced structure of TgCDPK6 was developed by a homology modeling method and was validated by PROCHECK, which showed that most amino acid residues were in the most favored regions. Using these analyses, 10 potential epitopes were predicted. The results indicated that TgCDPK6 could be a vaccine candidate antigen against T. gondii.