Identification of specific genes in Staphylococcus aureus strains associated with bovine mastitis
Identification of specific genes in Staphylococcus aureus strains associated with bovine mastitis
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DOI：
10.1016/j.vetmic.2010.03.020
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发表时间：
2010-10-26
影响因子：
3.3
通讯作者：
Ohlsen, Knut
中科院分区：
文献类型：
--
作者：
Kozytska, Svitlana;Stauss, Dennis;Ohlsen, Knut
金黄色葡萄球菌是引起奶牛乳房炎的常见病原，是奶牛业的主要经济损失。为了鉴定推定的牛特异性分子标记，对牛S. aureus菌株RF 122和52个先前测序的S.使用基因组查看器、注释工具Artemis Comparison Tool（ACT）、KEGG和NCBI BLAST数据库进行与人类感染相关的金黄色葡萄球菌分离株的分析。这导致了16个独特的RF122基因序列的鉴定，可用作区分牛与人菌株的分子标记。这些基因的分布进行了分析，在收集的牛乳腺炎菌株从荷兰和人类临床分离的PCR和Southern印迹。致病岛SaPIbov 3中只有4个基因（sab 1890、sab 1891、sob 1892、sab 1893）存在于大多数牛分离株中，但在人类临床S.金黄色葡萄球菌分离株这些结果表明，不存在所有牛S.金黄色葡萄球菌菌株，可以统一用作诊断标记基因。(C)2010 Elsevier B.V.保留所有权利。
Staphylococcus aureus is a common cause of bovine mastitis that is responsible for the main economic loss to the dairy industry. For identification of putative, bovine-specific molecular marker a genome comparison between bovine S. aureus strain RF122 and 52 previously sequenced S. aureus isolates associated with human infections using genome viewer, annotation tool Artemis Comparison Tool (ACT), KEGG and NCBI BLAST databases was carried out. This led to the identification of 16 unique RF122 gene sequences that may be used as molecular marker to distinguish bovine from human strains. The distribution of these genes was analyzed in a collection of bovine mastitis strains from the Netherlands and human clinical isolates by PCR and Southern blotting. Only four genes within the pathogenicity island SaPIbov3 (sab 1890, sab1891, sob 1892, sab1893) were present in the majority of isolates from cattle but were absent from human clinical S. aureus isolates. These results suggest that there is no gene/ORF uniformly shared by all bovine S. aureus strains that could be uniformly used as a diagnostic marker gene. (C) 2010 Elsevier B.V. All rights reserved.