Rostro-caudal variations in neuronal size reflect the topography of cellular phenotypes in the rat superior cervical sympathetic ganglion.
Rostro-caudal variations in neuronal size reflect the topography of cellular phenotypes in the rat superior cervical sympathetic ganglion.
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神经元大小的头尾变化反映了大鼠颈上交感神经节细胞表型的地形。
DOI：
10.1016/j.brainres.2005.07.029
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Horn,JohnP
中科院分区：
文献类型：
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作者：
Headley,DrewB;Suhan,NadineM;Horn,JohnP
哺乳动物颈上神经节（SCG）包含一个复杂的混合神经元表型，选择性地支配不同的外周目标。本研究通过分析细胞位置、大小与神经肽Y （NPY）、降钙素和降钙素基因相关肽（CGRP）免疫反应性表达的关系，研究了大鼠SCG交感神经表型的前尾侧形貌。我们观察到64%的SCG神经元表达NPY，整个神经节的平均直径为~ 24 μm。以往的研究表明，这些细胞大多具有血管收缩功能。相比之下，npy阴性神经元的大小从颈内神经附近的吻侧神经节的~ 25 μm到颈外神经和颈交感干之间的尾侧神经节的~ 30 μm不等。尾神经节中许多大的npy阴性神经元被密集的轴突篮包围，这些轴突篮对calretinin具有免疫反应，因此可能是分泌运动神经元，投射到唾液腺。与先前的报道一致，根据NPY的表达和与含有CGRP的纤维的接触，吻侧神经节含有少量的推定瞳孔运动神经元。目前的结果表明，神经元的大小可能为细胞识别提供了有用的帮助，特别是在尾神经节中，它们为哺乳动物SCG内的地形组织提供了进一步的证据。
The mammalian superior cervical ganglion (SCG) contains a complex mixture of neuronal phenotypes that selectively innervate different peripheral targets. The present study examined the rostro-caudal topography of sympathetic phenotypes in the rat SCG by analyzing the relation between cell position, size, and the expression of immunoreactivity for neuropeptide Y (NPY), calretinin, and calcitonin gene-related peptide (CGRP). We observed that 64% of SCG neurons expressed NPY and had an average diameter of ∼24 μm throughout the ganglion. Previous studies indicate that most of these cells are vasoconstrictor in function. By contrast, the size of NPY-negative neurons varied from ∼25 μm in the rostral ganglion near the internal carotid nerve to ∼30 μm in the caudal ganglion between the external carotid nerve and cervical sympathetic trunk. Many of the large NPY-negative neurons in the caudal ganglion were surrounded by dense axonal baskets that were immunoreactive for calretinin and therefore are likely to be secretomotor neurons projecting to salivary glands. Consistent with earlier reports, the rostral ganglion contained low numbers of presumptive pupillomotor neurons, based on their expression of NPY and contact with fibers containing CGRP. The present results indicate that neuronal size may provide a useful aid to cellular identification, especially in the caudal ganglion, and they provide further evidence of a topographic organization within the mammalian SCG.