A SIMPLE TECHNIQUE FOR SIMULTANEOUS HUMAN-LEUKOCYTES SEPARATION
A SIMPLE TECHNIQUE FOR SIMULTANEOUS HUMAN-LEUKOCYTES SEPARATION
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DOI：
10.1016/0022-1759(81)90087-9
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发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
NOGUEIRAMACHADO, JA
中科院分区：
文献类型：
--
作者：
BICALHO, HMS;GONTIJO, CM;NOGUEIRAMACHADO, JA
为了同时分离单核细胞、嗜中性粒细胞和嗜酸性粒细胞，我们将Thorsby和Bratlie（1970）和Heslop（1978）分离血细胞的技术进行了组合和改进，其基础是在多个不连续梯度上离心细胞。（A）15.0 ml 9.0% Ficoll（Sigma Chemical Company，St. Louis，MO）加10.0 ml 50.0% Hypaque（温斯洛普产品公司，纽约）（密度= 1.14）;（B）17.5 ml 9.0% Ficoll加10.0 ml 50.0% Hypaque（密度= 1.14）1.13）;（C）20.0 ml 9.0% Ficoll +10.0 ml 50.0% Hypaque（密度= 1.13）; 1.12）;和（D）24.0 ml 9.0% Ficoll加10.0 ml 33.9% Hypaque（密度= 1.08）。
We have combined and modified the techniques divised by Thorsby and Bratlie (1970) and Heslop (1978) for the separation of blood cells, based on the centrifugation of cells over a multiple discontinuous gradient, in order to obtain, simultaneously, the separation of mononuclear cells, neutrophils and eosinophils.The discontinuous gradient was prepared as described below from the following starting solutions:(A) 15.0 ml of 9.0% Ficoll (Sigma Chemical Company, St. Louis, MO) plus 10.0 ml of 50.0% Hypaque (Winthrop Products Inc., New York)(density= 1.14);(B) 17.5 ml of 9.0% Ficoll plus 10.0 ml of 50.0% Hypaque (dens.= 1.13);(C) 20.0 ml of 9.0% Ficoll plus 10.0 ml of 50.0% Hypaque (dens.= 1.12); and (D) 24.0 ml of 9.0% Ficoll plus 10.0 ml of 33.9% Hypaque (dens.= 1.08).