HUMAN C-MYB PROTOONCOGENE - NUCLEOTIDE-SEQUENCE OF CDNA AND ORGANIZATION OF THE GENOMIC LOCUS
HUMAN C-MYB PROTOONCOGENE - NUCLEOTIDE-SEQUENCE OF CDNA AND ORGANIZATION OF THE GENOMIC LOCUS
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DOI：
10.1073/pnas.83.24.9636
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
DALLAFAVERA, R
中科院分区：
文献类型：
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作者：
MAJELLO, B;KENYON, LC;DALLAFAVERA, R
我们已经分离出含有.apprxeq的人c-myb mRNA的cDNA克隆。.apprxeq.的3.4千碱基。3.8千碱基mRNA序列。核苷酸序列分析表明，c-myb mRNA包含1920个核苷酸的开放阅读框，可编码一个72kda的蛋白。c-myb蛋白的cDNA核苷酸序列和预测氨基酸序列与鸡和小鼠的相应蛋白高度同源。特别是，该蛋白的NH2端含有51个残基的3倍串联重复区域是进化保守的，并且是唯一与果蝇c-myb蛋白同源的区域。这个区域可能代表一个重要的功能结构，最有可能是dna结合结构域。cDNA克隆已被用于分离基因组克隆和确定c-myb基因的初步内含子/外显子组织。5”和3”编码外显子和非编码外显子的鉴定表明，人类c-myb基因座跨越了一个40千碱基的区域。
We have isolated cDNA clones of the human c-myb mRNA that contain .apprxeq. 3.4 kilobases of the .apprxeq. 3.8-kilobase mRNA sequence. Nucleotide sequence analysis shows that the c-myb mRNA contains an open reading frame of 1920 nucleotides, which could encode a 72-kDa protein. The cDNA nucleotide sequence and the predicted amino acid sequence of the c-myb protein are highly homologous to the corresponding chicken and mouse proteins. In particular, a region toward the NH2 terminus of the protein containing a 3-fold tanden repeat of 51 residues is evolutionarily conserved and is the only region of homology with the Drosophila c-myb protein. This region may represent a functionally important structure, most likely the DNA-binding domain. cDNA clones have been used to isolate genomic clones and to define a preliminary intron/exon organization of the c-myb gene. Identification of 5'' and 3'' coding and noncoding exons indicates that the human c-myb locus spans a 40-kilobase region.