Involvement of lymphocytes in dextran sulfate sodium-induced experimental colitis
Involvement of lymphocytes in dextran sulfate sodium-induced experimental colitis
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DOI：
10.3748/wjg.v12.i2.302
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发表时间：
2006-01-14
影响因子：
4.3
通讯作者：
Oh, Kwon Ik
中科院分区：
文献类型：
--
作者：
Kim, Tae Woon;Seo, Jae Nam;Oh, Kwon Ik
目的：探讨淋巴细胞在葡聚糖硫酸钠诱导的结肠炎中的作用。方法：用不同剂量的葡聚糖硫酸钠(DSS)诱导野生型B6对照和RAG-1基因敲除(H-2(B)单倍型)小鼠结肠炎，从症状和组织学指标(如体重减轻、存活率、腹泻严重程度、结肠长度不足和组织学改变)评价结肠炎的发生。结果：尽管高剂量(5%)DSS处理的RAG-1基因敲除小鼠的结肠炎进展与B6对照组小鼠相似，但RAG-1基因敲除小鼠的结肠炎进展比小剂量(1.5%)DSS处理的B6小鼠更容易耐受。RAG-1基因敲除小鼠的症状参数和组织病理学改变均得到改善。结论：在低剂量DSS刺激下，淋巴细胞的存在有助于结肠炎的进展。淋巴细胞可能在DSS诱导的结肠炎中起加重作用。(C)2006年《假发出版社》。版权所有。
AIM: To investigate the roles of lymphocytes in the development of dextran sulfate sodium-induced colitis.METHODS: Using various doses of dextran sulfate sodium (DSS), we induced colitis in wild-type B6 control and Rag-1 knockout (H-2(b) haplotype) mice, and evaluated the colitis in terms of symptomatic and histologic parameters, such as weight loss, survival, severity of diarrhea, shortage of colon length and histological changes. Symptomatic parameters were checked daily and histological changes were scored.RESULTS: Although development of colitis in Rag-1 knockout mice treated with high dose (5%) of DSS was comparable to that in B6 control mice, colitis progression was much more tolerable in Rag-1 knockout mice compared to than in B6 mice treated with low dose (1.5%) DSS. Symptomatic parameters as well as histopathologic changes were improved in Rag-1 knockout mice.CONCLUSION: These results indicate that the presence of lymphocytes contributes to colitis progression at low dose of DSS stimulation. Lymphocytes may play roles as an aggravating factor in DSS-induced colitis. (c) 2006 The WIG Press. All rights reserved.