Quinoxaline-Based Polymer Dots with Ultrabright Red to Near-Infrared Fluorescence for In Vivo Biological Imaging
Quinoxaline-Based Polymer Dots with Ultrabright Red to Near-Infrared Fluorescence for In Vivo Biological Imaging
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DOI：
10.1021/jacs.5b06710
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发表时间：
2015-08-19
影响因子：
15
通讯作者：
Chant, Yang-Hsiang
中科院分区：
文献类型：
--
作者：
Liu, Hong-Yi;Wu, Pei-Jing;Chant, Yang-Hsiang
本文描述了基于喹啉的半导体聚合物点（Pdots）的设计和合成，具有近红外荧光、超高亮度、大斯托克斯位移和出色的细胞靶向能力。我们还在聚合物骨架中引入了氟原子和长烷基链，系统地研究了它们对Pdots荧光量子产率的影响。这些新的喹诺啉基Pdots的荧光量子产率高达47%，Stokes位移大于150 nm。单粒子分析表明，Pdots的平均每粒子亮度至少比市售量子点高6倍。我们进一步证明了这种基于喹诺沙林的新型Pdots用于有效和特异性的细胞和亚细胞标记，而没有任何明显的非特异性结合。此外，我们还对Pdots在HeLa细胞和斑马鱼胚胎上的细胞毒性进行了评价，证明了Pdots具有良好的生物相容性。利用其极高的亮度和最小的细胞毒性，我们首次使用Pdots对活的斑马鱼胚胎进行了体内微血管成像。这些基于喹诺啉的nir荧光Pdots有望在各种体外和体内生物学研究中得到广泛应用。
This article describes the design and synthesis of quinoxaline-based semiconducting polymer dots (Pdots) that exhibit near-infrared fluorescence, ultrahigh brightness, large Stokes shifts, and excellent cellular targeting capability. We also introduced fluorine atoms and long alkyl chains into polymer backbones and systematically investigated their effect on the fluorescence quantum yields of Pdots. These new series of quinoxaline-based Pdots have a fluorescence quantum yield as high as 47% with a Stokes shift larger than 150 nm. Single-particle analysis reveals that the average per-particle brightness of the Pdots is at least 6 times higher than that of the commercially available quantum dots. We further demonstrated the use of this new class of quinoxaline-based Pdots for effective and specific cellular and subcellular labeling without any noticeable nonspecific binding. Moreover, the cytotoxicity of Pdots were evaluated on HeLa cells and zebrafish embryos to demonstrate their great biocompatibility. By taking advantage of their extreme brightness and minimal cytotoxicity, we performed, for the first time, in vivo microangiography imaging on living zebrafish embryos using Pdots. These quinoxaline-based NIR-fluorescent Pdots are anticipated to find broad use in a variety of in vitro and in vivo biological research.