Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats.
Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats.
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DOI：
10.1016/s0278-6915(00)00011-9
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发表时间：
2000-05
期刊：
影响因子：
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通讯作者：
Chau-Jong Wang;Jin-Ming Wang;Wea-lung Lin;C. Chu;F. Chou;T. Tseng
中科院分区：
文献类型：
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作者：
Chau-Jong Wang;Jin-Ming Wang;Wea-lung Lin;C. Chu;F. Chou;T. Tseng
木槿花青素 (HA) 是一种存在于木槿花干花中的天然色素，木槿花是一种当地的软饮料材料和药草，对其抗氧化生物活性进行了研究。初步研究表明HAs能够消除1,1-二苯基-2-三硝基苯肼自由基。使用叔丁基过氧化氢 (t-BHP) 诱导的大鼠原代肝细胞细胞毒性和大鼠肝毒性模型进一步评估了这种抗氧化生物活性。结果表明，浓度为 0.10 和 0.20 mg/ml 的 HA 显着减少了 t-BHP (1.5 mm) 处理 30 分钟诱导的乳酸脱氢酶的泄漏和丙二醛的形成。体内研究表明，在单剂量t-BHP（0.2 mmol/kg，ip）之前口服HAs（100和200 mg/kg）预处理5天可显着降低肝酶标志物（丙氨酸和天冬氨酸转氨酶）的血清水平，并减少氧化性肝损伤。肝脏的组织病理学评估表明，木槿花色素可降低大鼠肝脏损伤的发生率，包括炎症、白细胞浸润和 t-BHP 引起的坏死。根据上述结果，我们推测芙蓉色素可能在预防生命系统氧化损伤方面发挥作用。
Hibiscus anthocyanins (HAs), a group of natural pigments occurring in the dried flowers of Hibiscus sabdariffa L., which is a local soft drink material and medical herb, were studied for antioxidant bioactivity. The preliminary study showed that HAs were able to quench the free radicals of 1,1-diphenyl-2-picrylhydrazyl. This antioxidant bioactivitiy was further evaluated using the model of tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity in rat primary hepatocytes and hepatotoxicity in rats. The results demonstrated that HAs, at the concentrations of 0.10 and 0.20 mg/ml, significantly decreased the leakage of lactate dehydrogenase and the formation of malondialdehyde induced by a 30-min treatment of t-BHP (1.5 mm). The in vivo investigation showed that the oral pretreatment of HAs (100 and 200 mg/kg) for 5 days before a single dose of t-BHP (0.2 mmol/kg, ip) significantly lowered the serum levels of hepatic enzyme markers (alanine and aspartate aminotransferase) and reduced oxidative liver damage. The histopathological evaluation of the liver revealed that Hibiscus pigments reduced the incidence of liver lesions including inflammatory, leucocyte infiltration, and necrosis induced by t-BHP in rats. Based on the results described above, we speculate that Hibiscus pigments may play a role in the prevention of oxidative damage in living systems.