Bioactive poly(L-lactic acid)-chitosan hybrid scaffolds
Bioactive poly(L-lactic acid)-chitosan hybrid scaffolds
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DOI：
10.1016/j.msec.2008.03.005
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
J. Mano;G. Hungerford;J. Ribelles
中科院分区：
文献类型：
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作者：
J. Mano;G. Hungerford;J. Ribelles
通过将 CHT 沉积到孔壁上来制备聚（L-乳酸）/壳聚糖（PLLA/CHT）混合支架，以便在先前制备的 PLLA 支架内形成微结构的低密度泡沫。通过扫描电子显微镜（SEM）和微型计算机断层扫描（micro-CT）评估支架的形态和孔结构。 CHT 部分的微观结构取决于其在用于浸渍 PLLA 支架的溶液中的浓度。 CHT 部分的吸水能力允许在混合结构的结构中产生磷酸钙前体，将它们浸入交替的磷和钙溶液中。生物活性测试使用模拟体液中的培养方案，离子浓度大约等于人体血浆的离子浓度，可以观察混合支架内的磷灰石沉积，尤其是 CHT 含量较高的支架。这项工作表明，通过遵循简单快速的预钙化方案，将壳聚糖与 PLLA 多孔结构相结合，可以获得具有用于骨组织工程潜力的生物活性支架。
Poly(L-lactic acid)/Chitosan (PLLA/CHT) hybrid scaffolds were prepared by depositing CHT onto the pore walls in order to form a micro-structured low dense foam inside previously prepared PLLA scaffolds. The morphology and pore architecture of the scaffolds were evaluated by scanning electronic microscopy (SEM) and micro-computational tomography (micro-CT). The microstructure of the CHT fraction depends on its concentration in the solution used to impregnate the PLLA scaffolds. The water-uptake capability of the CHT fraction permitted the production of calcium-phosphate precursors in the structure of the hybrid constructs, on dipping them into alternate phosphorous and calcium solutions. Bioactivity tests, using incubation protocols in a simulated body fluid with ion concentrations approximately equal to those of human blood plasma, allowed the observation of the apatite deposition within the hybrid scaffolds, especially the ones with a higher CHT content. This work shows that bioactive scaffolds, with potential to be used in bone tissue engineering, can be obtained by combining chitosan with PLLA porous structures, following a simple and fast pre-calcification protocol.