An RNA-Seq transcriptome analysis revealing novel insights into aluminum tolerance and accumulation in tea plant
An RNA-Seq transcriptome analysis revealing novel insights into aluminum tolerance and accumulation in tea plant
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RNA-Seq 转录组分析揭示了茶树铝耐受性和积累的新见解
DOI：
10.1007/s00425-017-2688-6
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发表时间：
2017-07-01
期刊：
影响因子：
4.3
通讯作者：
Ni, Dejiang
中科院分区：
文献类型：
--
作者：
Li, Yong;Huang, Jie;Ni, Dejiang
主要结论茶树（Camellia sinensis L.）O. Kuntze）是一种高铝耐性和富集植物。基于从头转录组分析，组装了茶树耐铝相关候选基因。茶树与水稻、拟南芥、荞麦等植物具有相同的耐铝机制，茶树除了具有较强的耐铝性外，还表现出较好的耐铝性能，并在叶片中积累了较高的铝含量，但没有表现出明显的毒害症状。因此，铝是茶树的超积累元素和有益元素。然而，茶树耐铝和积累铝的全基因组分子机制尚不清楚。在这项研究中，评估了梯度Al水平暴露后通过RNA-Seq进行的转录组分析，以进一步揭示所涉及的候选基因。共产生了超过4.68亿个高质量的read，并重新组装了213，699个unigenes，其中8922个unigenes在所使用的7个数据库中全部注释。大量的转运蛋白、转录因子、细胞色素P450、泛素连接酶、有机酸合成、热激蛋白在高铝胁迫下差异表达（P≤ 0. 05），这些蛋白可能是铝胁迫下植物耐铝或积累铝的理想候选蛋白。此外，一些候选的铝响应基因相关的铝螯合，细胞壁修饰和有机酸排泄已经很好地阐明了已经发现在拟南芥，水稻，荞麦。因此，一些一致的耐铝机制，跨物种的指示。总之，转录组数据提供了有用的见解，有前途的候选人，进一步表征基因的功能，参与茶树耐铝和积累。
Main conclusionThe tea plant (Camellia sinensisL. O. Kuntze) is a high aluminum (Al) tolerant and accumulator species. Candidate genes related to Al tolerance in tea plants were assembled based on de novo transcriptome analysis. The homologs implied some common and distinct Al-tolerant mechanism between tea plants and rice, Arabidopsis and buckwheat.In addition to high Al tolerance, the tea plant exhibits good performance exposure to a proper Al level, and accumulates high Al in the leaves without any toxicity symptom. Therefore, Al was considered as a hyperaccumulator and beneficial element for tea plants. However, the whole-genome molecular mechanisms accounting for Al-tolerance and accumulation remain unknown in tea plants. In this study, transcriptome analysis by RNA-Seq following a gradient Al-level exposure was assessed to further reveal candidate genes involved. Totally more than 468 million high-quality reads were generated and 213,699 unigenes were de novo assembled, among which 8922 unigenes were all annotated in the seven databases used. A large number of transporters, transcription factors, cytochrome P450, ubiquitin ligase, organic acid biosynthesis, heat shock proteins differentially expressed in response to high Al (P≤ 0.05) were identified, which were most likely ideal candidates involved in the Al tolerance or accumulation. Furthermore, a few of the candidate Al-responsive genes related to Al sequestration, cell wall modification and organic acid excretion have been well elucidated as was already found in Arabidopsis, rice, and buckwheat. Thus, some consistent Al-tolerance mechanisms across the species are indicated. In conclusion, the transcriptome data provided useful insights of promising candidates for further characterizing the functions of genes involved in Al tolerance and accumulation in tea plants.