A recombinant envelope protein vaccine against West Nile virus
A recombinant envelope protein vaccine against West Nile virus
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DOI：
10.1016/j.vaccine.2005.03.006
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发表时间：
2005-06-10
期刊：
影响因子：
5.5
通讯作者：
Koski, RA
中科院分区：
文献类型：
--
作者：
Ledizet, M;Kar, K;Koski, RA
西尼罗河病毒（WN）是一种黄病毒，1999年首次出现在北美。自那时以来，美国已有600多人死亡和22，000匹马感染该病毒。我们在果蝇S2细胞中表达了截短形式的WN病毒包膜（E）蛋白。这种可溶性重组E蛋白被来自自然感染马的抗体识别，表明它含有天然表位。用重组E蛋白与氢氧化铝联合免疫小鼠和马产生高滴度抗体。免疫小鼠对致命病毒剂量的攻击具有抗性。免疫马的血清，给予幼稚小鼠，赋予对致命的WN病毒攻击的抵抗力。此外，免疫马的血清在体外中和西尼罗河病毒，如空斑减少试验所示。这种重组形式的E蛋白，结合氢氧化铝，是一种候选疫苗，可以保护人类和马免受WN病毒感染。(c)2005爱思唯尔有限公司保留所有权利。
West Nile (WN) virus is a flavivirus that first appeared in North America in 1999. Since then, more than 600 human deaths and 22,000 equine infections have been attributed to the virus in the United States. We expressed a truncated form of WN virus envelope (E) protein in Drosophila S2 cells. This soluble recombinant E protein was recognized by antibodies from naturally infected horses, indicating that it contains native epitopes. Mice and horses produced high-titer antibodies when immunized with recombinant E protein combined with aluminum hydroxide. Immunized mice were resistant to challenge with a lethal viral dose. Sera from immunized horses, administered to naive mice, conferred resistance against a lethal WN viral challenge. In addition, sera of immunized horses neutralized West Nile virus in vitro, as demonstrated by plaque reduction assays. This recombinant form of E protein, combined with aluminum hydroxide, is a candidate vaccine that may protect humans and horses against WN virus infections. (c) 2005 Elsevier Ltd. All rights reserved.