Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs
Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs
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吉氏巴贝虫感染犬血清中抗吉氏巴贝虫热休克蛋白70抗体和抗犬热休克蛋白70抗体的检测
DOI：
10.1016/j.vetpar.2011.03.006
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发表时间：
2011
影响因子：
2.6
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Yamasaki M;Nakamura K;et al
在急性和慢性B的血清中检测到识别吉氏巴氏杆菌或犬红细胞（RBC）70千道尔顿（kDa）蛋白的抗体。吉布森感染在这些血清中，与重组B. gibsoni和犬热休克蛋白70（rBgHsp 70和rcHsp 70）;因此，B。gibsoni和犬RBC 70-kDa蛋白似乎分别是BgHsp 70和cHsp 70。在受感染的狗中，这些抗体的量在感染后增加。有趣的是，在两只家兔中产生的针对rBgHsp 70的多克隆抗体不仅与rBgHsp 70反应，而且与来自犬RBC的rcHsp 70和天然cHsp 70反应。由于BgHsp 70与cHsp 70具有较高的同源性（70.8%），因此抗rBgHsp 70抗体可能与cHsp 70发生交叉反应。此外，BgHsp 70和cHsp 70的定位通过间接荧光测定来观察。结果，在红细胞的膜表面上未发现cHsp 70，表明红细胞不是抗cHsp 70抗体的靶。同时，抗rBgHsp 70抗体仅对红细胞外寄生虫染色。这一结果表明，BgHsp 70将在红细胞外期的寄生虫表面表达。这些结果表明BgHsp 70是犬B中的高免疫原性蛋白。gibsoni感染，红细胞外寄生虫可能是抗BgHsp 70抗体的靶点。
Antibodies that recognized either Babesia gibsoni or canine red blood cell (RBC) 70-kilodalton (kDa) protein were detected in serum from acutely and chronically B. gibsoni-infected. In those sera, antibodies that reacted with recombinant B. gibsoni and canine heat shock protein 70 (rBgHsp70 and rcHsp70) were detected; therefore, B. gibsoni and canine RBC 70-kDa proteins seemed to be BgHsp70 and cHsp70, respectively. In infected dogs, the amounts of these antibodies increased after infection. Interestingly, polyclonal antibody raised against rBgHsp70 in two rabbits reacted not only with rBgHsp70 but also with rcHsp70 and native cHsp70 from canine RBCs. Because BgHsp70 showed high homology with cHsp70 (70.8%), anti-rBgHsp70 antibody might cross-react with cHsp70. Additionally, the localizations of both BgHsp70 and cHsp70 were observed by indirect fluorescence assay. As a result, cHsp70 was not found on the membrane surface of erythrocytes, suggesting that erythrocytes would not be targets of anti-cHsp70 antibody. Meanwhile, only exoerythrocytic parasites were stained by anti-rBgHsp70 antibody. This result showed that BgHsp70 would be expressed on the surface of parasites during the exoerythrocytic stage. These results indicated that BgHsp70 was a highly immunogenic protein in canine B. gibsoni infection, and that exoerythrocytic parasites might be targets of anti-BgHsp70 antibody.