Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase
Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase
复制标题
域间通讯调节亮氨酰-tRNA合成酶的tRNA依赖性预转移编辑
DOI：
10.1042/bj20121258
复制
发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Wang, En-duo
中科院分区：
文献类型：
--
作者：
Tan, Min;Zhu, Bin;Wang, En-duo
EcLeuRS [大肠杆菌LeuRS（亮氨酰-tRNA合成酶）]已经进化出依赖tRNA的转移前和转移后编辑能力，以确保催化特异性。这两种编辑功能都依赖于tRNA CCA尾进入LeuRS酶的编辑结构域，根据X射线晶体结构研究，这导致合成和编辑结构域之间的界面的动态无序取向。本研究的结果表明，这种tRNA触发的构象重排导致编辑和合成结构域之间通过它们的界面进行结构域间的通信，并且这种通信机制调节tRNA依赖的转移前编辑的活性。此外，tRNA依赖性编辑反应抑制了合成活性位点的非同源氨基酸的误激活。这些结果也表明了一种新的质量控制机制的EcLeuRS，这是通过协调之间的合成和编辑结构域。
EcLeuRS [Escherichia coli LeuRS (leucyl-tRNA synthetase)] has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity. Both editing functions rely on the entry of the tRNA CCA tail into the editing domain of the LeuRS enzyme, which, according to X-ray crystal structural studies, leads to a dynamic disordered orientation of the interface between the synthetic and editing domains. The results of the present study show that this tRNA-triggered conformational rearrangement leads to interdomain communication between the editing and synthetic domains through their interface, and this communication mechanism modulates the activity of tRNA-dependent pre-transfer editing. Furthermore, tRNA-dependent editing reaction inhibits misactivating non-cognate amino acids from the synthetic active site. These results also suggested a novel quality control mechanism of EcLeuRS which is achieved through the co-ordination between the synthetic and editing domains.