Quantitative analysis of three-dimensional morphology and membrane dynamics of red blood cells during temperature elevation
Quantitative analysis of three-dimensional morphology and membrane dynamics of red blood cells during temperature elevation
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DOI：
10.1038/s41598-019-50640-z
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发表时间：
2019-10-01
影响因子：
4.6
通讯作者：
Moon, Inkyu
中科院分区：
文献类型：
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作者：
Jaferzadeh, Keyvan;Sim, MinWoo;Moon, Inkyu
红细胞的最佳功能与周围环境密切相关。本研究分析了健康红细胞（RBC）在不同温度下膜轮廓、平均红细胞血红蛋白（MCH）和细胞膜波动（CMF）的变化。在不到一小时的时间内将温度从17摄氏度升高到41摄氏度，并通过离轴配置记录全息图。全息图重建后，我们提取单个红细胞，并评估其形态相关的功能（投影表面积和球度系数），MCH，和CMF。我们观察到，升高温度会导致三维（3D）轮廓发生变化。由于CMF振幅与红细胞膜的弯曲曲率高度相关，温度引起的形状变化会改变CMF的图和振幅;主要是在较高的温度下，酒窝区域出现较大的波动。无论形状变化如何，MCH均未随温度变化而改变。
The optimal functionality of red blood cells is closely associated with the surrounding environment. This study was undertaken to analyze the changes in membrane profile, mean corpuscular hemoglobin (MCH), and cell membrane fluctuations (CMF) of healthy red blood cells (RBC) at varying temperatures. The temperature was elevated from 17 degrees C to 41 degrees C within a duration of less than one hour, and the holograms were recorded by an off-axis configuration. After hologram reconstruction, we extracted single RBCs and evaluated their morphologically related features (projected surface area and sphericity coefficient), MCH, and CMF. We observed that elevating the temperature results in changes in the three-dimensional (3D) profile. Since CMF amplitude is highly correlated to the bending curvature of RBC membrane, temperature-induced shape changes can alter CMF's map and amplitude; mainly larger fluctuations appear on dimple area at a higher temperature. Regardless of the shape changes, no alterations in MCH were seen with temperature variation.