Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing
Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing
复制标题
454焦磷酸测序揭示银鲫肠道微生物群及其起源
DOI：
10.1007/s11274-013-1322-4
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发表时间：
2013-09-01
影响因子：
4.1
通讯作者：
Wang, Gui-Tang
中科院分区：
文献类型：
--
作者：
Wu, Shan-Gong;Tian, Jing-Yun;Wang, Gui-Tang
The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order:Veilonellasp., Lachnospiraceae, Lactobacillales,Streptococcussp., andLactobacillussp.). The second most abundant OTU wasRothiasp. (Actinobacteria). The most likely potential probiotics (Lactobacillussp., andBacillussp.) and opportunists (Aeromonassp., andAcinetobactersp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.
