Genomic Analysis of European Drosophila melanogaster Populations Reveals Longitudinal Structure, Continent-Wide Selection, and Previously Unknown DNA Viruses
Genomic Analysis of European Drosophila melanogaster Populations Reveals Longitudinal Structure, Continent-Wide Selection, and Previously Unknown DNA Viruses
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DOI：
10.1093/molbev/msaa120
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发表时间：
2020-09-01
影响因子：
10.7
通讯作者：
Gonzalez, Josefa
中科院分区：
文献类型：
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作者：
Kapun, Martin;Barron, Maite G.;Gonzalez, Josefa
遗传变异是进化的燃料，长期遗传变异对短期进化和局部适应特别重要。迄今为止，自然种群遗传变异时空模式的研究一直具有挑战性，因为全面采样在后勤上很困难，而且整个种群的测序成本高昂。在这里，我们解决这些问题，使用协作的方法，测序48汇集人口样本从32个地点，并进行第一次全大陆的基因组分析欧洲果蝇的遗传变异。我们的分析揭示了纵向人口结构，提供了证据，整个大陆范围内的选择性扫描，确定候选基因的地方气候适应，并在染色体倒位和转座因子频率的文件倾斜。我们还描述了苍蝇微生物组组成的种群间差异，并在我们的样本中鉴定出五种新的DNA病毒。
Genetic variation is the fuel of evolution, with standing genetic variation especially important for short-term evolution and local adaptation. To date, studies of spatiotemporal patterns of genetic variation in natural populations have been challenging, as comprehensive sampling is logistically difficult, and sequencing of entire populations costly. Here, we address these issues using a collaborative approach, sequencing 48 pooled population samples from 32 locations, and perform the first continent-wide genomic analysis of genetic variation in European Drosophila melanogaster. Our analyses uncover longitudinal population structure, provide evidence for continent-wide selective sweeps, identify candidate genes for local climate adaptation, and document clines in chromosomal inversion and transposable element frequencies. We also characterize variation among populations in the composition of the fly microbiome, and identify five new DNA viruses in our samples.