DIFFERENTIAL DISTRIBUTION OF FACTORS INVOLVED IN PRE-MESSENGER-RNA PROCESSING IN THE YEAST-CELL NUCLEUS
DIFFERENTIAL DISTRIBUTION OF FACTORS INVOLVED IN PRE-MESSENGER-RNA PROCESSING IN THE YEAST-CELL NUCLEUS
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DOI：
10.1128/mcb.10.7.3524
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发表时间：
1990-07-01
影响因子：
5.3
通讯作者：
SPECTOR, DL
中科院分区：
文献类型：
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作者：
POTASHKIN, JA;DERBY, RJ;SPECTOR, DL
先前通过多种显微技术已表明酵母细胞核被分为两个区域。我们使用对小核核糖核酸（snRNAs）的2,2,7 - 三甲基鸟苷帽结构以及小核核糖核蛋白颗粒的一种蛋白质成分具有特异性的抗体，来确定小核核糖核蛋白颗粒在酵母细胞核内的分布。这些研究是利用裂殖酵母粟酒裂殖酵母（Schizosaccharomyces pombe）和芽殖酵母酿酒酵母（Saccharomyces cerevisiae）进行的。通过免疫荧光显微镜和免疫电子显微镜观察，大多数丰富的snRNAs定位于细胞核中此前被称为核仁的部分。snRNAs的这种分布与在哺乳动物细胞中发现的不同，这表明酵母细胞核的核仁部分除了那些与RNA聚合酶I活性相关的区域外，还包含其他功能域。
The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a protein component of small nuclear ribonucleoprotein particles to identify the distribution of small nuclear ribonucleoprotein particles within the yeast cell nucleus. These studies were performed with the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae. By using immunofluorescence microscopy and immunoelectron microscopy, most of the abundant snRNAs were localized to the portion of the nucleus which has heretofore been referred to as the nucleolus. This distribution of snRNAs is different from that found in mammalian cells and suggests that the nucleolar portion of the yeast nucleus contains functional domains in addition to those associated with RNA polymerase I activity.