Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants
Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants
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DOI：
10.1007/s12274-020-2700-5
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发表时间：
2020-02
期刊：
影响因子：
9.9
通讯作者：
Xinzhou Wu;Run Qin;Hanxiang Wu;Guangkai Yao;Yue Zhang;Ping Li;Yizhu Xu;Zhi-xiang Zhang;
中科院分区：
文献类型：
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作者：
Xinzhou Wu;Run Qin;Hanxiang Wu;Guangkai Yao;Yue Zhang;Ping Li;Yizhu Xu;Zhi-xiang Zhang;
设计和发现载体介导的修饰农药对于减少农药用量和提高农药利用率至关重要，但由于对植物转运机制的了解有限，这仍然是一个巨大的挑战。纳米结构/纳米粒子辅助激光解吸/电离技术已成为生物组织分析的首选分析工具，而植物科学中的潜在应用由于无法切片植物叶片和花瓣而受到阻碍。在这里，我们报道了金纳米颗粒(AuNP)-浸泡纸印迹质谱仪成像(MSI)用于植物叶片中农药转运的时空可视化。这种方法在保留空间信息和提高农药的电离效率方面具有双重作用，而不考虑由于均匀的AuNP沉积而产生的成像伪影。利用这一MSI平台，我们首次提出了目前鲜为人知的农用化学品植物转运机制。载体介导的农药的动态过程可以清楚地显示出来，包括转运体穿过质膜，通过韧皮部向下转运到茎中，扩散到木质部，反过来，在处理后的叶片边缘积累。此外，这种AuNP辅助的纸张印记方法可以与基于激光的MSI仪器高度兼容，加速广泛领域的研究，特别是在纳米材料开发和生命科学方面。
Design and discovery of carrier-mediated modified pesticides are vital for reducing pesticide dosage and increasing utilization, yet it remains a great challenge due to limited insights into plant translocation mechanisms. Nanostructure/nanoparticle assisted laser desorption/ionization strategy has established itself as a preferential analytical tool for biological tissue analysis, whereas potential applications in plant sciences are hindered with regard to the inability to slice plant leaves and petals. Herein, we report gold nanoparticle (AuNP)-immersed paper imprinting mass spectrometry imaging (MSI) for the spatiotemporal visualization of pesticide translocation in plant leaves. This approach plays a dual role in preserving spatial information and improving ionization efficiency for pesticides regardless of imaging artifacts due to homogenous AuNP deposition. Using this MSI platform, we proposed the elaborate plant translocation mechanism of agrochemicals for the first time, which is currently poorly understood. The dynamic processes of carrier-mediated pesticides can be clearly visualized, including crossing of plasma membranes by transporters, translocation downward in stems through the phloem, diffusion to the xylem and, conversely, accumulation at margins of the treated leaves. Moreover, this AuNP-assisted paper imprinting method could be highly compatible with laser-based MSI instruments, expediting researches across a broad range of fields, especially in nanomaterial development and life sciences.