The scaffold protein JIP3 functions as a downstream effector of the small GTPase ARF6 to regulate neurite morphogenesis of cortical neurons
The scaffold protein JIP3 functions as a downstream effector of the small GTPase ARF6 to regulate neurite morphogenesis of cortical neurons
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DOI：
10.1016/j.febslet.2010.05.020
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发表时间：
2010-07-02
期刊：
影响因子：
3.5
通讯作者：
Kanaho, Yasunori
中科院分区：
文献类型：
--
作者：
Suzuki, Atsushi;Arikawa, Chihiro;Kanaho, Yasunori
小GTP酶ADP-核糖基化因子6（ARF 6）在多种细胞功能中起着至关重要的作用。为了更好地了解ARF 6介导的信号传导和细胞功能的分子机制，我们在小鼠大脑中寻找新的ARF 6结合蛋白。我们确定了信号支架蛋白JNK相互作用蛋白3（JIP 3），它只在神经元中表达，作为ARF 6的下游效应子。一个独特的显性负突变的ARF 6，这是无法与JIP 3相互作用，并敲低小鼠皮层神经元中的JIP 3的过表达刺激神经突起的伸长和分支。这些结果为ARF 6/JIP 3信号调节神经突形态发生提供了证据。
The small GTPase ADP-ribosylation factor 6 (ARF6) plays crucial roles in a wide variety of cell functions. To better understand the molecular mechanisms of ARF6-mediated signaling and cellular functions, we sought new ARF6-binding proteins in the mouse brain. We identified the signaling scaffold protein JNK-interacting protein 3 (JIP3), which is exclusively expressed in neurons, as a downstream effector of ARF6. Overexpression of a unique dominant negative mutant of ARF6, which was unable to interact with JIP3, and knockdown of JIP3 in mouse cortical neurons stimulated the elongation and branching of neurites. These results provide evidence that ARF6/JIP3 signaling regulates neurite morphogenesis.