Fourier transform infrared analysis of the photosynthetic oxygen-evolving center
Fourier transform infrared analysis of the photosynthetic oxygen-evolving center
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DOI：
10.1016/j.ccr.2007.05.001
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发表时间：
2008-02
影响因子：
20.6
通讯作者：
T. Noguchi
中科院分区：
文献类型：
--
作者：
T. Noguchi
傅里叶变换红外（FTIR）差谱是研究酶活性中心结构的有效手段。它基本上可以检测到所有参与触发反应的分子，所获得的结构信息与X射线晶体学的结构信息是互补的。光合放氧中心（OEC），其中包括嵌入在光系统II蛋白质复合物的Mn 4Ca簇的光诱导FTIR差光谱，获得的光谱变化后的第一个S1-到-S2过渡或在S-状态循环。通过同位素取代、量子化学计算和定点诱变进行谱带分配，同时通过对OEC施加一定的扰动并检测伴随的光谱变化来分析OEC的结构和反应。本文综述了OEC的红外光谱研究，以了解光合放氧的分子机制。
Fourier transform infrared (FTIR) difference spectroscopy is a powerful method to study the detailed structures of active sites in enzymes. It can detect basically all molecules involved in trigger reactions and the structural information obtained is complementary to that by X-ray crystallography. Light-induced FTIR difference spectra of the photosynthetic oxygen-evolving center (OEC), which consists of the Mn4Ca cluster embedded in photosystem II protein complexes, were obtained as spectral changes upon the first S1-to-S2transition or during the S-state cycle. Band assignments were performed by isotopic substitution, quantum chemical calculations, and site-directed mutagenesis, while the structures and reactions of OEC were analysed by applying certain perturbations on the OEC and detecting concomitant spectral changes. This review summarizes the FTIR studies on OEC thus far performed for understanding the molecular mechanism of photosynthetic oxygen evolution.