JUN-B DIFFERS IN ITS BIOLOGICAL PROPERTIES FROM, AND IS A NEGATIVE REGULATOR OF, C-JUN
JUN-B DIFFERS IN ITS BIOLOGICAL PROPERTIES FROM, AND IS A NEGATIVE REGULATOR OF, C-JUN
复制标题
DOI：
10.1016/0092-8674(89)90754-x
复制
发表时间：
1989-12-22
期刊：
影响因子：
64.5
通讯作者：
KARIN, M
中科院分区：
文献类型：
--
作者：
CHIU, R;ANGEL, P;KARIN, M
c - Jun、Jun - B和Jun - D蛋白作为同二聚体或Jun - Fos异二聚体与佛波酯应答元件（TRE）结合。我们证明，c - Jun和Jun - B在激活AP - 1应答基因的能力上存在显著差异。c - Jun是含有单个TRE的c - jun和胶原酶启动子的有效激活因子，而Jun - B不是。此外，Jun - B抑制c - Jun对这些启动子的激活。另一方面，与c - Jun一样，Jun - B是含有多聚TRE构建体的有效激活因子。通过使用嵌合蛋白，我们表明c - Jun和Jun - B的不同行为是由于它们的激活结构域存在差异。Jun - B的反式激活依赖于相邻结合因子之间的协同相互作用，而c - Jun的激活不需要这种相互作用。这种差异行为极大地扩展了Jun家族的调控潜能。
c-Jun, Jun-B, and Jun-D proteins bind to the TPA response element (TRE) either as homodimers or as Jun-Fos heterodimers. We demonstrate that c-Jun and Jun-B nevertheless differ markedly in their ability to activate AP-1 responsive genes. c-Jun is an efficient activator of the c-jun and collagenase promoters, which contain a single TRE; Jun-B is not. Furthermore, Jun-B inhibits activation of these promoters by c-Jun. On the other hand, like c-Jun, Jun-B is an efficient activator of constructs containing multimeric TREs. Using chimeric proteins, we show that the distinct behavior of c-Jun and Jun-B is due to differences in their activation domains. Trans-activation by Jun-B depends on cooperative interactions between adjacently bound factors, while activation by c-Jun does not require such interactions. This differential behavior greatly expands the regulatory potential of the Jun family.