Fabrication and Characterization of Highly Porous Chitosan/Poly(DL lactic-co-glycolic acid) Nanocomposite Scaffolds Using Electrospinning and Freeze Drying
Fabrication and Characterization of Highly Porous Chitosan/Poly(DL lactic-co-glycolic acid) Nanocomposite Scaffolds Using Electrospinning and Freeze Drying
复制标题
利用静电纺丝和冷冻干燥制备高孔壳聚糖/聚（DL 乳酸-乙醇酸）纳米复合支架的制备和表征
DOI：
10.1166/jbmb.2014.1444
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发表时间：
2014-06-01
影响因子：
0.5
通讯作者：
Shen, Changyu
中科院分区：
文献类型：
--
作者：
Cui, Zhixiang;Zhao, Haibin;Shen, Changyu
目前，具有理想纳米形貌的三维（3-D）支架的制造仍然是一个重大的挑战和活跃的研究领域。在这项研究中，一个高度多孔的，3-D壳聚糖/聚（DL-乳酸-共-乙醇酸）（PLGA）纳米复合材料支架具有交错的PLGA纳米纤维壳聚糖孔相结合的静电纺丝和单向冷冻干燥技术。用扫描电镜（SEM）对壳聚糖/PLGA纳米复合支架的孔结构进行了表征。为了控制管状形态和纳米形貌，不同的工艺参数，如壳聚糖溶液的浓度和静电纺丝时间，已被广泛研究。研究了壳聚糖/PLGA纳米复合支架的孔隙率、吸水率、压缩模量和压缩强度等性能。结果表明，壳聚糖/PLGA纳米复合支架可形成特殊的三维管状多孔结构。壳聚糖/PLGA纳米复合支架的孔隙率和吸水率随着壳聚糖溶液浓度的增加和静电纺丝时间的延长而降低。此外，由于PLGA纳米纤维的加入，壳聚糖/PLGA纳米复合支架的压缩模量和压缩强度增加。
At present, the fabrication of three-dimensional (3-D) scaffolds possessing desirable nanotopography remains a significant challenge and an active research area. In this study, a highly porous, 3-D chitosan/poly(DL-lactic-co-glycolic acid) (PLGA) nanocomposite scaffolds featuring chitosan pores with interlaced PLGA nanofibers were produced by combining electrospinning and unidirectional freeze drying techniques. The porous structures of chitosan/PLGA nanocomposite scaffolds were characterized by scanning electron microscopy (SEM). In order to control the tubular morphology and nanotopography, different processing parameters, such as the concentration of chitosan solution and electrospinning time, have been extensively studied. The properties of chitosan/PLGA nanocomposite scaffolds, including the porosity, water absorption, modulus of compressibility, and compression strength, were also investigated. The results showed that a special 3-D tubular porous structure with PLGA nanofibers could be formed in the chitosan/PLGA nanocomposite scaffolds. The porosity and water absorption of the chitosan/PLGA nanocomposite scaffolds decreased with an increase in chitosan solution concentration and electrospinning time. In addition, the modulus of compressibility and the compressive strength of the chitosan/PLGA nanocomposite scaffolds increased due to the addition of PLGA nanofibers.