Surface Fluctuations and Coalescence of Nucleolar Droplets in the Human Cell Nucleus.
Surface Fluctuations and Coalescence of Nucleolar Droplets in the Human Cell Nucleus.
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DOI：
10.1103/physrevlett.121.148101
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发表时间：
2018-10-05
影响因子：
8.6
通讯作者：
Zidovska A
中科院分区：
文献类型：
--
作者：
Caragine CM;Haley SC;Zidovska A
核仁是嵌入在细胞核内染色质溶液中的无膜细胞器。通过分析活体人核仁的表面动力学和融合动力学，我们发现核仁表面表现出微妙但可测量的形状波动，连接两个融合核仁的颈部半径以r(T)∼T1/2的形式增长。这与低表面张力的∼10−6 Nm−1液滴在高粘度∼103PAS的外部流体中聚合是一致的。我们的研究提出了一种非侵入性的方法，使用自然探针及其动力学来研究细胞及其成分的材料属性。
The nucleolus is a membraneless organelle embedded in chromatin solution inside the cell nucleus. By analyzing surface dynamics and fusion kinetics of human nucleoli in vivo, we find that the nucleolar surface exhibits subtle, but measurable, shape fluctuations and that the radius of the neck connecting two fusing nucleoli grows in time as r(t) ∼ t1/2. This is consistent with liquid droplets with low surface tension ∼ 10−6 Nm−1 coalescing within an outside fluid of high viscosity ∼ 103 Pas. Our study presents a noninvasive approach of using natural probes and their dynamics to investigate material properties of the cell and its constituents.