Resonance Raman spectra of myoglobins reconstituted with spirographis and isospirographis hemes and iron 2,4-diformylprotoporphyrin IX. Effect of formyl substitution at the heme periphery.
Resonance Raman spectra of myoglobins reconstituted with spirographis and isospirographis hemes and iron 2,4-diformylprotoporphyrin IX. Effect of formyl substitution at the heme periphery.
复制标题
用螺旋线虫和等螺旋线虫血红素以及 2,4-二甲酰基原卟啉 IX 铁重建的肌红蛋白的共振拉曼光谱。
DOI：
10.1021/bi00543a020
复制
发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
T. Kitagawa
中科院分区：
文献类型：
--
作者：
M. Tsubaki;K. Nagai;T. Kitagawa
Motonari Tsubaki、1 Kiyoshi Nagai 和 Teizo Kitakawa** 摘要：合成了 Spirographis 和 isospirographis 血红素和铁 2, 4-二甲酰基原卟啉 IX，并通过将它们合并到抹香鲸无肌红蛋白 (Mb) 中来观察它们的共振拉曼光谱，以保护它们免受光降解。重建的 Mb 在还原态和氧化态，甚至在铁低自旋态下均在 1650-1670 cm" 1 附近表现出甲酰基 C= 0 伸缩带，尽管据报道该带对于细胞色素氧化酶的细胞色素 a 是不可观察到的。C= 0 伸缩频率清楚地反映了脱辅基蛋白血红素腔中卟啉环的位置 2 和 4 的不等价性。天然脱氧-Mb 在 342 和 242 cm" 1 处的拉曼谱线在甲酰基取代时显示出频移，并分别分配给卟啉 v8 和 vl7 模式。卟啉 CaCm 伸缩 (10) 和 C „N 伸缩频率 (v4) 虽然敏感性较低，但随取代位置的不同而不同。
Motonari Tsubaki, 1 Kiyoshi Nagai, and Teizo Kitagawa** abstract: Spirographis and isospirographis hemes and iron 2, 4-diformylprotoporphyrin IX were synthesized and their resonance Raman spectra were observed by incorporating them into sperm whale apomyoglobin (Mb) to protect them from photodegradation. The reconstituted Mb’s exhibited the formyl C= 0 stretching band around 1650-1670 cm" 1 both in reduced and in oxidized states and even in the ferric low-spin state, although the band is reported to be unobservable for cytochrome a of cytochrome oxidase. The C= 0 stretching frequencies clearly reflected nonequivalence of positions 2 and 4 of the porphyrin ring in the heme cavity of apoprotein. The Raman lines of native deoxy-Mb at 342 and 242 cm" 1 showed a frequency shift upon formyl substitution and were assigned to the porphyrin v8 and vl7 modes, respectively. The porphyrin CaCm stretching (10) and, though less sensitively, C „N stretching frequencies (v4) differed with the substituted pos-