Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries
Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries
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DOI：
10.1016/j.jprot.2012.02.020
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发表时间：
2012-04-18
影响因子：
3.3
通讯作者：
Righetti, Pier Giorgio
中科院分区：
文献类型：
--
作者：
Esteve, Clara;D'Amato, Alfonsina;Righetti, Pier Giorgio
描述了不同类型的提取方案用于鉴定橄榄（油橄榄）种子和果肉中的蛋白质，通过采用常规提取方法和用ProteoMiner捕获以及在pH 7.4和pH 2.2下用自制的组合肽配体文库（HM-CPLL）捕获。由于CPLL的使用，能够显着放大低丰度物种的信号，已经确定了相当多的化合物：种子中有61种（目前文献中只有4种），果肉中有231种（到目前为止描述了56种），这是迄今为止对橄榄蛋白质组最深入的研究。在种子中，它突出了种子贮藏蛋白、油质蛋白和组蛋白的存在。在果肉中，过敏性索马汀样蛋白（Ole e 13）被确认为橄榄果肉中最丰富的蛋白质。目前的研究也已经进行，目的是确定蛋白质在橄榄油和确定种子和果肉蛋白质的相对贡献，如果有的话，在油中的存在。(C)2012 Elsevier B. V.保留所有权利。
Different types of extraction protocols are described for identifying proteins in seed and pulp of olive (Olea europea), by employing both conventional extraction methods and capture with ProteoMiner as well as with in house-made combinatorial peptide ligand libraries (HM-CPLLs) at pH 7.4 and at pH 2.2. Thanks to the use of CPLLs, able to dramatically amplify the signal of low-abundance species, a quite large number of compounds has been indeed identified: 61 in the seed (vs. only four reported in current literature) and 231 in the pulp (vs. 56 described so far), the deepest investigation up to the present of the olive proteome. In the seed, it highlights the presence of seed storage proteins, oleosins and histones. In the pulp, the allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp. The present research has also been undertaken with the aim of identifying proteins in olive oil and ascertaining the relative contribution of seed and pulp proteins in their presence, if any, in oils. (C) 2012 Elsevier B.V. All rights reserved.