Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress
Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress
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循环往复剪切应力下红细胞的动态变形能力
DOI：
10.1109/iembs.2004.1404401
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Takatani
中科院分区：
文献类型：
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作者：
N. Watanabe;T. Yasuda;H. Kataoka;S. Takatani
心血管器械中的红细胞（RBC）暴露于来自各个方向的不同程度的剪切应力。然而，红细胞在这种剪切应力下的变形能力或变形能力还没有很好地理解。在这项研究中，我们设计并建立了一个系统，可以诱导循环往复剪切应力的红细胞悬浮液。将循环往复剪切应力装置的臂连接到平行玻璃板的上片，在平行玻璃板之间含有人RBC的悬浮液（在32重量%葡聚糖磷酸盐缓冲溶液中稀释的1%血细胞比容全血）。使用连接到偏心凸轮-马达系统的滑块-曲柄轴机构产生3.0mm冲程长度的上玻璃板的循环往复运动。马达的每一次旋转在滑块的向前和向后方向上产生各3.0mm的行程。将两个玻璃板之间的间隙调节至30微米。将循环往复玻璃板装置连接到光学显微镜载物台（带有x40物镜透镜的IX 71 Olympus），用于用350瓦金属卤化物光源照明。将高速照相机（MEMREMCAM fx-K3 Nac，5000帧/秒，快门保持打开）连接到显微镜以捕获RBC在循环剪切应力下的变形过程。初步结果表明，最大切应力的幅值与红细胞的变形能力相关。这表明循环往复装置在评估红细胞破坏前变形性的时间响应方面具有潜在的应用价值。未来的研究将集中在红细胞相对于往复板速度的相对速度的研究。
Red blood cells (RBCs) in the cardiovascular devices are exposed to varying degree of the shear stress from all the directions. However the RBCs' deformability or the deformation capability under such a shear stress is not well understood. In this study, we designed and built a system that can induce a cyclically reciprocating shear stress to a RBC suspension. The arm of the cyclically reciprocating shear stress device was attached to the upper piece of the parallel glass plates between which a suspension of human RBCs (1% hematocrit whole blood diluted in a 32 weight% dextran phosphate buffer solution) was contained. The cyclic reciprocating motion of the upper glass plate of 3.0 mm stroke length was produced using a slider-crank shaft mechanism that was linked to an eccentric cam-motor system. Each rotation of the motor produced a 3.0 mm stroke each in the forward and backward direction of the slider block. The clearance between the two glass plates was adjusted to 30 micrometer. The cyclic reciprocating glass plate apparatus was attached to a light microscope stage (IX71 Olympus with x40 objective lens) for illumination with a 350 watt metal halide light source. A high speed camera (MEMREMCAM fx-K3 Nac, 5000 frames per second with shutter kept open) was attached to the microscope to capture the deformation process of the RBCs under cyclic shear stress. The preliminary result indicated that the correlation between the amplitude of the maximum shear stress and the RBCs' deformability. This indicates a potential application of the cyclic reciprocating device to evaluate the temporal response of the RBCs deformability prior to its destruction. The future study will focus on the study of the relative velocity of the erythrocytes with respect to the velocity of the reciprocating plate.