S-antigen-specific rat T cell lines recognize peptide fragments of S-antigen and mediate experimental autoimmune uveoretinitis and pinealitis.
S-antigen-specific rat T cell lines recognize peptide fragments of S-antigen and mediate experimental autoimmune uveoretinitis and pinealitis.
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DOI：
10.4049/jimmunol.136.8.2875
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发表时间：
1986-04
影响因子：
4.4
通讯作者：
D. Gregerson;W. F. Obritsch;S. Fling;J. Cameron
中科院分区：
文献类型：
--
作者：
D. Gregerson;W. F. Obritsch;S. Fling;J. Cameron
从视网膜S抗原免疫的Lewis大鼠的脾细胞和淋巴结细胞中分离出两种表达T辅助细胞表面表型的S抗原特异性大鼠T细胞系（W 3/25+，OX 8-），其中一种既没有表现出实验性自身免疫性葡萄膜视网膜炎的临床症状，也没有表现出组织病理学症状。另一只大鼠在分离细胞系之前已经从严重的实验性自身免疫性葡萄膜视网膜炎中恢复了 2 个月。这两种细胞系都对组织相容性抗原呈递细胞呈递的 S 抗原具有特异性，并且在体外对牛视网膜 S 抗原的溴化氰裂解产生的几种肽有反应。静脉注射引起的损伤1 至 10 X 10(6) 个活细胞系的转移涉及视网膜和松果体，正如用免疫致病剂量的 S 抗原对 Lewis 大鼠进行免疫时所发现的那样。眼睛和松果体的组织学检查揭示了实验性自身免疫性葡萄膜视网膜炎的典型病理损伤，包括淋巴细胞和炎症细胞对视网膜和周围组织以及松果体的明显浸润。能够介导自身免疫性疾病的 T 细胞明显存在，并且很容易从无症状和恢复期动物中分离出来。从这两只大鼠分离的T细胞系中，对S抗原溴化氰肽的特异性或细胞表面表型没有发现显着差异，并且从原始大鼠中获取的血清抗体对S抗原溴化氰肽的特异性或效价也没有发现任何差异。
Two S-antigen-specific rat T cell lines expressing the T helper cell surface phenotype (W 3/25+, OX 8-) have been isolated from the spleen and lymph node cells of retinal S-antigen-immunized Lewis rats, one of which displayed neither clinical nor histopathologic signs of experimental autoimmune uveoretinitis. The other rat had recovered from severe experimental autoimmune uveoretinitis for 2 mo before isolation of the cell line. Both lines are specific for S-antigen presented by histocompatible antigen-presenting cells, and also respond in vitro to several of the peptides produced by cyanogen bromide cleavage of bovine retinal S-antigen. The lesions induced by the i.v. transfer of from 1 to 10 X 10(6) viable line cells involve the retina and pineal gland, as is found when Lewis rats are immunized with immunopathogenic doses of S-antigen. Histologic examination of the eyes and pineal glands revealed pathologic lesions typical of experimental autoimmune uveoretinitis, and consisted of marked infiltration of the retina and surrounding tissues and the pineal gland by lymphocytes and inflammatory cells. T cells capable of mediating autoimmune disease are clearly present and readily isolated from both asymptomatic and convalescent animals. No significant differences in specificity for the cyanogen bromide peptides of S-antigen or cell surface phenotype were found in the T cell lines isolated from these two rats, nor was any difference found in the specificity or titer of serum antibodies taken from the original rats for the cyanogen bromide peptides of S-antigen.