In vivo γ-rays induced initial DNA damage and the effect of famotidine in mouse leukocytes as assayed by the alkaline comet assay
In vivo γ-rays induced initial DNA damage and the effect of famotidine in mouse leukocytes as assayed by the alkaline comet assay
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DOI：
10.1269/jrr.06055
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发表时间：
2007-03-01
影响因子：
2
通讯作者：
Haeri, S. Abolghasem
中科院分区：
文献类型：
--
作者：
Mozdarani, Hossein;Nasirian, Borzo;Haeri, S. Abolghasem
γ辐射/DNA损伤/小鼠白细胞/彗星试验/法莫替丁电离辐射诱导DNA的各种损伤，每种损伤都可用作生物剂量测定或物质辐射防护效应研究的生物指示剂。为了评估不同剂量的γ射线在小鼠白细胞中体内诱导的DNA损伤和法莫替丁的作用，在用4戈伊γ射线照射后的不同时间间隔从Balb/c雄性小鼠收集血液。为了评估反应，用1至4格雷剂量的γ射线照射小鼠。照射前不同时间间隔腹腔注射法莫替丁5 mg/kg。从每个样品制备四个载玻片，并使用标准方案进行碱性彗星试验。结果表明，辐射以剂量依赖方式显著增加白细胞DNA损伤（p
Gamma irradiation/DNA damage/Mouse leukocytes/Comet assay/Famotidine.Ionizing radiation induces a variety of lesions in DNA, each of which can be used as a bio-indicator for biological dosimetry or the study of the radioprotective effects of substances. To assess gamma ray-induced DNA damage in vivo in mouse leukocytes at various doses and the effect of famotidine, blood was collected from Balb/c male mice after irradiation with 4 Gy gamma-rays at different time intervals post-irradiation. To assess the response, mice were irradiated with doses of gamma-rays at I to 4 Grays. Famotidine was injected intra-peritoneally (i.p) at a dose of 5 mg/kg at various time intervals before irradiation. Four slides were prepared from each sample and alkaline comet assay was performed using standard protocols. Results obtained show that radiation significantly increases DNA damage in leukocytes in a dose dependent manner (p