The enantioselective toxicity and oxidative stress of beta-cypermethrin on zebrafish.
The enantioselective toxicity and oxidative stress of beta-cypermethrin on zebrafish.
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DOI：
10.1016/j.envpol.2017.05.088
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发表时间：
2017-10
影响因子：
8.9
通讯作者：
Xiyan Mu;Gongming Shen;Ying Huang;Jianbo Luo;Lizhen Zhu;Suzhen Qi;Yingren Li;Chengju Wang
中科院分区：
文献类型：
--
作者：
Xiyan Mu;Gongming Shen;Ying Huang;Jianbo Luo;Lizhen Zhu;Suzhen Qi;Yingren Li;Chengju Wang
尽管近年来高效氯氰菊酯对水生生物的毒性已成为一个重要的关注，但其对非靶标生物的对映选择性影响却知之甚少。为了研究β-内酰胺对斑马鱼的对映选择性毒性，将成年斑马鱼暴露于一系列等体积浓度的4种β-内酰胺对映体和β-内酰胺对映体96 h。此外，在环境相关剂量（0.01和0.1 μg/L）下，测定了β-内酰胺对映体和外消旋体暴露15和30 d后斑马鱼肝和脑中4种抗氧化酶的活性和丙二醛（MDA）含量。急性毒性实验结果表明，1 R-顺式-α和1 R-反式-α对映体的致死率高于1 S-顺式-α兰德和1 S-反式-αR。在0.1 μg/L浓度下，1 R-cis-α S、1 R-transs-α S对映体和β-甘草酸均能引起大鼠肝脏MDA含量的升高，而只有1 R-cis-α S引起脑脂质过氧化反应。1 R-顺式-α和1 R-反式-α对映体和β-甘草酸对斑马鱼肝和脑中抗氧化酶水平有明显的调节作用。与此相反，在测试浓度下暴露于1 S-顺式-α兰德和1 S-反式-α雷对映体的斑马鱼中未观察到显著的氧化应激。这项工作证明了β-环糊精对成年斑马鱼的毒性和氧化应激存在对映选择性，这在环境风险评估中应予以考虑。
Although the toxicity of beta-cypermethrin (beta-CYP) to aquatic organisms has become a significant concern in recent years, its enantioselective effects on non-target organisms is poorly understood. To investigate the enantioselective toxicity of beta-CYP on zebrafish, adult zebrafish were exposed to a series of isometric concentrations of four beta-CYP enantiomers and the beta-CYP racemate for 96 h. In addition, the activities of four antioxidant enzymes and the malondialdehyde (MDA) content in zebrafish liver and brain were tested after 15 and 30 days beta-CYP enantiomers and racemate exposure under environmentally relevant dosages (0.01 and 0.1 μg/L). According to the acute toxicity results, the 1R-cis-αSand 1R-trans-αSenantiomers were more lethal than 1S-cis-αRand 1S-trans-αR. At 0.1 μg/L, the 1R-cis-αSand 1R-trans-αSenantiomers, and the beta-CYP racemate could significantly induce a hepatic MDA content at 30 days post exposure (dpe), while only 1R-cis-αScaused brain lipid peroxidation. An apparent regulation of antioxidase levels was observed in zebrafish liver and brain after exposure to the 1R-cis-αSand 1R-trans-αSenantiomers, and the beta-CYP racemate. In contrast, no significant oxidative stress was observed in zebrafish exposed to 1S-cis-αRand 1S-trans-αRenantiomers under the test concentrations. This work demonstrated the occurrence of enantioselectivity in toxicity and oxidative stress of beta-CYP to adult zebrafish, which should be considered in environmental risk assessments.